After obtaining a PhD degree, Dejan Gmajner was employed in economy, in AKVAMI Ltd. company.
F.07 Improvements to an existing product
Organization of international school Electroporation based Technologies and Treatments in 2011, 2012 and 2013. At the practiacal work of school we included electroporation of planar lipid bilayers and molecular dynamic simulations of membrane electroporation.
B.01 Organiser of a scientific meeting
The nanosecond electroporation of vesicles in cells and mitohondria was studied. The results showed that the position of vesicles and mitochondria does not have a considerable impact on its ITV unless the vesicles (or mitochondria) are positioned close to the nucleus. A more pronounced effect on electroporation of vesicles was observed when electrical parameters of the model e.g. medium and cytoplasm conductivity were reduced. By comparing the responses to 50 ns and 10 ns pulse we also demonstrated that shorter pulses are less damaging to the cell membrane, therefore providing a higher chance for selective electroporation of vesicles. However, our calculations show that selective electroporation of smaller, 50 nm vesicles (archaeosomes) is highly unlikely as the cell membrane and membranes of other organelles are electroporated as well, regardless of the medium conductivity, cytoplasm conductivity and internal archaeosome conductivity. Our results indicate that for electroporation of archaeosomes the pulse duration should be reduced as possible and the pulse amplitude should be carefully considered.
F.35 Other
COBISS.SI-ID: 8803668Cell membrane of strictly aerobic hyperthermophilic Archaeon Aeropyrum pernix K1 consists of a unique combination of lipids, which enables it to survive at temperatures over 100°C. Arheosomes prepared from purified archaeal C25, 25 lipids were characterized physicochemically, showing a great stability to the different environmental effects (temperature, pH). We examined the effects archaeal C25, 25 lipids on selected human cell lines in vitro, through cytotoxicity and interaction of lipid vesicles with cells.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 259208960