The paper presents the system for measuring planar lipid bilayer properties. The system is composed of a control unit, an output stage, LCR meter, pumps for filling reservoirs, a bath with temperature regulation and a measurement chamber with four electrodes. The planar lipid bilayer is automatically formed by folding method on apertures of different sizes. The automatization is assured by two syringes which are clamped in actuators. Actuators are driven and controlled by control unit via RS-232 communication. The temperature of planar lipid bilayer can be regulated between 15 °C and 55 °C. The regulation is assured by insertion of measurement chamber into the temperature regulated bath. Different shapes of voltage or current clamp signals can be applied to planar lipid bilayer. By measuring the planar lipid bilayer response to applied signal, planar lipid bilayer capacitance and breakdown voltage can be determined. The cutoff frequencies of the system output stage are 11 kHz and 17 kHz for voltage clamp method and current clamp method respectively.
COBISS.SI-ID: 9398356
The influence of pH (6.0, 7.0, 8.0) of the growth medium of Aeropyrum pernix K1 on the structural organization and fluidity of archaeosomes prepared from a polar-lipid methanol fraction (PLMF) was investigated using fluorescence anisotropy and electron paramagnetic resonance (EPR) spectroscopy. Both of these methods indicate that the pH of the growth medium of A. pernix does not significantly influence its average membrane fluidity. These results are in accordance with TLC analysis of isolated lipids, which show no significant differences between PLMF isolated from A. pernix grown in medium with different pH.
COBISS.SI-ID: 4073848
Giant vesicles (GVs) constituted of different species of archaebacterial diether phospholipids and glycolipids were prepared by electroformation and observed under a phase contrast and/or fluorescence microscope. Archaebacterial lipids and different mixtures of archaebacterial and standard lipids formed GVs.
COBISS.SI-ID: 4113016