In this work, the current knowledge on the role of bacteriocins of lactic acid bacteria in the intestinal tract in terms of enteric cytoprotection was reviewed. Special attention was devoted to bacteriocins of established probiotic bacteria exhibiting an activity against gastrointestinal pathogenic bacteria. The possible direct effects of bacteriocins as immunomodulators or signal moleculs on the host organism has been also discussed taking into account the fact that there is limited evidence. Since this topic is very challenging and relatively new the work represents a valuable contribution.
This paper describes the microbiological part of a research of the role of human milk in development of breast fed child's intestinal microbiota. For the study of microbiota in samples of colostrum, milk, meconium and feces of infants combined genetic and conventional microbiological methods are used. Analyses are focused on the important group of lactic acid bacteria, bifidobacteria, staphylococci and streptococci. Qualitative methods such as DGGE and T-RFLP are used to study the diversity of the microbiota and real-time PCR for the quantification of the selected bacterial groups.