The polymerase chain reaction (PCR) has become a powerful diagnostic tool for the analysis of microorganisms in different types of samples including faeces. Faecal samples are a difficult specimen for the application in PCR due to the presence of PCR inhibitors. The paper describes an efficient method for DNA extraction based on the use of hydrophilic magnetic microspheres (poly 2-hydroxyethyl methacrylate-co-glycidyl methacrylate). It can be efficiently used for DNA isolation from different types of environmental and clinical samples, including faecal samples.
COBISS.SI-ID: 3012744
The guidelines are for healthy full-term infants. Exclusive breastfeeding is recommended during the first 6 months of life. After the first week of life, we recommend the addition of vitamin D (400 IE/day) for breastfed as well as formula fed infants. Introduction of complementary feeding is recommended not before the 17th and not after the 26th week of life. The introduction of gluten recommend between 6 and 7 months of life, while breast-feeding, because the introduction of gluten during breastfeeding reduces the risk of celiac disease, type 1 diabetes and allergy to wheat.
COBISS.SI-ID: 4368703
A book chapter presents a comprehensive review of the use of PCR method in different samples, which is the result of many years experience of our research group using molecular methods in analysis of food and biological samples. The methodology described in the work is applicable not only to dairy products and probiotic products in general only, but also for the analysis of biological samples such as human milk or faecal samples.
COBISS.SI-ID: 3079304