In our study, the in vitro and in vivo tests were performed to demonstrate that in the mammary gland of goats are progenitor cells, or cells similar to stem cells. In the clonogenic assay for the detection of proliferating progenitor cells were goat epithelial cells plated in low numbers on collagen-coated plates with addition of mitomicin C inactivated fibroblasts. Colonies that grew, were characterized morphologically and with immunofluorescence. Bazed on the expression of the line-specific cytokeratins 14 and 18, we determined the frequency of individual progenitor cells. The content of progenitor cells was dependent on the tested sample and amounted to: (i) 20-70% for luminale ancestry, (ii) 20-80% for myoepithelial and (iii) up to 20% for bipotent progenitor cells. In vivo evidence for the presence of stem cell was regeneration of epithelial structures in collagen gels. Because we are still searching for antibodies for sorting epithelial cells, we used in preliminary tests unsorted cells, which should include some stem cells. The cells were mixed with inactivated fibroblasts and collagen immobilized in gels and placed under the kidney capsule of mice NOD / SCID. After 4-6 weeks we removed the implants and performed paraffin fixation. Immunofluorescently stained structures that resemble the intact epithelium in the goat mammary gland were found, where the CK14-positive cells cover luminalne CK18-positive luminal cells, which open to the lumen. Thus we have proved that in the goat mammary gland cells exist that are capable of proliferation and regeneration.
B.03 Paper at an international scientific conferenceCOBISS.SI-ID: 2951304
Paper describes the results of the transcriptomic analysis of the goat mammary epithelial cells after infection with Mycoplasma agalactiae. Transcriptomic analysis was performed in three time intervals after infection (3, 12 and 24 hours PI). Total, we identified over 1500 differentially expressed genes belonging to different metabolic pathways, including the cell cycle, immune response, energy metabolism and nucleic acid metabolism. The study contributes to a better understanding of developments in the mammary gland after infection and opens new possibilities for developing new therapeutic and preventive strategies.
B.03 Paper at an international scientific conferenceCOBISS.SI-ID: 2811528