We describe the development of a quick test for the detection of the quarantine viroid PSTVd. The test provides results in typically 20-25 minutes and is specific to the target. It is also easily amenable to on-site testing.
COBISS.SI-ID: 2683727
Fire blight, a devastating disease of pome fruit trees continues to pose threat to agricultural production. Detection of its causative agent, bacterium Erwinia amylovora, is usually straightforward in symptomatic samples. Methods with increased sensitivity however, are sometimes needed for detection of E. amylovora and real-time PCR assays have been shown to have required sensitivity and reliability. Here we summarize our previous results on real-time PCR detection of fire blight and present new, fast and sensitive real-time PCR assay.
COBISS.SI-ID: 2522447