The aim of our study was to investigate the effect of vitamin E, Sweet chestnut wood extract (EX-TAN) and their combination on oxidative stress in vivo and oxidative stability of meat in chickens. Fifty chickens were individually caged and divided into five groups (N=10). Negative control group (K-PALM) received diet enriched with palm fat, positive control group (K-LAN) and other experimental group received diets ennriched with linseed oil. Experimental groups were enriched to contain in total 85 IU vitamin E/kg (VITE) or 3 g EX-TAN.kg (TAN) or 85 IU vitamin E/kg (VITE) plus 3 g EX-TAN/kg (VITE+TAN). We studied the extent of DNA damage, the information of lipid oxidation products (MDA) in blood plasma, liver and breast muscle, total, antioxidant status, antioxidant capacity of lipid soluble compounds, vitamin E in plasma, liver and breast meat and fatty acid composition of meat. Vitamin E was not able to prevent all harmful effects of oxidative damage. Also it was not able to totally improved stability of meat. The EX-TAN had no impact on oxidative stress, except on lymphocyte DNA damage. Furthermore, it did not improve meat lipid stability. The combination of both additives significantly improved some markers of oxidative stress (concentrations of [alfa]-tocopherol and stability of meat) to significantly better extent, what shows synergistic action of vitamin E and EX-TAN.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 2962824The aim of the study was to evaluate the effectiveness of α- (α-TOC) and γ-tocopherol (γ-TOC) to prevent lipid peroxidation in muscles of chickens fed diets containing 5 % of n-3 PUFA rich linseed oil. The fatty acid composition, α- and γ-tocopherol content, and malondialdehyde concentrations (MDA) of breast and thigh muscles were measured. Additionally we measured the overall vitamin E isomer distribution, oxidative status and liver gene expression. In thigh muscle, but not in breast muscle we found higher proportion of long chain n-3 fatty acids in groups fed antioxidants compared to Cont+, however overall PUFA percentage in thigh was lower than in breast. Tocopherol concentrations reflected the feeding regimen and were also higher in thigh muscles. The retention of α-TOC was much bigger than of γ-TOC. The gene expression analyses are showing that feeding PUFA affected mostly genes involved in lipid metabolism and regulation of oxidative stress, while α-TOC was involved in cholesterol metabolism and γ-TOC in immune processes. These results indicate that feeding linseed oil increases PUFA concentrations in meat but also increases lipid oxidation. α-TOC is able to prevent lipid oxidation while γ-TOC is not, so their combination is only partially effective. The difference in action between α-TOC and γ-TOC is also evident in differential liver gene expression.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 3005320