We aimed to determine differentially expressed protein spots in the leaves of drought stressed plants of the varieties Starozagorski čern and Tiber compared to control plants. Protein samples from drought stressed and control plants were labelled with different fluorescent dyes and were separated using two-dimensional gel electrophoresis (2-DE). Protein spots of interest were selected for further analysis with LC-MS/MS using Progenesis SameSpot software. Significance testing was performed by partial least square regression analysis.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 3921000Aquaporins are cell membrane proteins that exhibit water-transporting ability and express differentially in drought. They have regulation properties and multiple integrated functions in plant growth, development and adaptation to environmental stress such as salinity and drought. Using PCR approach, we cloned several aquaporin gene-like sequences from bean genome that were of app. 500 – 1000 nucleotides in length. With the comparison of bean translated nucleotide sequences with previously identified plant aquaporins, using blast algorithms in BLAST search tool, we successfully classified them as PIP and TIP members having conserved motifs (e.g. NPA box). Phylogenetic analyses revealed less than 24% of amino acid similarity between PIP and TIP subfamilies and several distinguished groups within subfamilies. Aminoacid diversity of bean aquaporin gene-like sequences was higher within PIP group. In the present study novel bean, aquaporin gene-like sequences are characterized, that are important for tagging the potential targets for breeding of drought tolerant bean.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 3919976