This article describes the annotation of gene clusters for biosynthesis of natural products, containing modular biosynthetic enzymes polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) in the genome of S. tsukubaensis, recently sequenced by Acies Bio. The analysis of these very large genes in genomes obtained by pyrosequencing is demanding as gene clusters are distributed among several contigs, sequencing errors can introduce apparent frame-shifts and in addition, these genes often contain very long repetitive regions. The article describes the analysis of the genome by phylogenetic approach and the ClustScan program, which enabled the identification of 4 PKS gene clusters and 6 NRPS gene clusters in the genome of S. tsukubaensis. In addition, PCR and sequencing enabled us to correct apparent frame-shifts and RT-PCR approach was used to demonstrate active transcription of one of the identified gene clusters.
COBISS.SI-ID: 4142456