Interactions between bacteria and material surfaces play an important role in biology and different process technologies. The objective of this study is to investigate the influence of surface roughness on the degree of bacterial adhesion. The surface roughness is adjusted as needed by polishing the glass plates made of borosilicate. The roughness, morphology and texture of the glass surface was measured and evaluated by Atomic force microscopy (AFM). The adhered cells are stained and detected spectrophotometrically and consequently compared with the results obtained by scanning electron microscopy. The experiments were made with Pseudomonas aeruginosa. Staining of the adhered cells on glass plates shows that adhesion of bacteria P. aeruginosa greatly depends on the surface characteristics. The adhesion capacity increases with increasing roughness of the glass surface. We had been shown that P. aeruginosa better occupy rough surfaces than smooth surfaces. Preliminary results indicate that the bacterial adhesion depends on the surface roughness.
B.06 Other
COBISS.SI-ID: 4431211