Like in the previous year one of the lab works was made available to the students attending the international scientific workshop and postgraduate course Electroporation based technologies and treatments COST TD1104. The course was dedicated to inactivation of bacteria by irreversible electroporation. The protocol is based on the results of this project.
B.01 Organiser of a scientific meeting
COBISS.SI-ID: 269548544Infection with C. difficile have a major impact on public health, and therefore there are a number of initiatives towards effective infection control and new management options. This was also aimed at the symposium entitled "The Importance of frequency and control of infections with the bacterium Clostridium difficile", which took place on 18 April 2013, starting at 13 hours at Brdo near Kranj (Kokra Hotel, Hall Elegans). The symposium was organized by one of the project partners.
B.01 Organiser of a scientific meeting
COBISS.SI-ID: 16908566Changes of bacterial and viral populations were monitored monthly for the duration of one year in the effluent of Domžale-Kamnik waste water treatment plant (WWTP). The composition of entire bacterial population was determined by denaturing high performance liquid chromatography (DHPLC) of amplified 16S rDNA gene. Additionally, bacteria from genus Clostridium were cultivated and strains identified as C. difficile were further typed into ribotypes. Viral populations of rotaviruses, astroviruses, noroviruses GG I and II, sapoviruses and hepatitis A virus were analysed after concentration of 5l volumes with CIM-QA 8ml tube columns (BIA separations) colums, followed by real time quantitative RT-qPCR detection. Our results show that the overall bacterial population in WWTP effluent varies in time, but the bacterial and viral intestinal pathogens are present throughout the year.
F.01 Acquisition of new practical knowledge, information and skills
COBISS.SI-ID: 512356664