Waterborne infections have been shown to be important in outbreaks of gastroenteritis throughout the world. Although improved sanitary conditions are being progressively applied, fecal contaminations remain an emerging problem also in developed countries. The aim of our study was to investigate the prevalence of fecal contaminated water sources in Slovenia, including surface waters and groundwater sources throughout the country. In total, 152 water samples were investigated, of which 72 samples represents groundwater from individual wells, 17 samples from public collection supplies and 63 samples from surface stream waters. Two liters of untreated water samples werecollected and concentrated by the adsorption/elution technique with positively charged filters followed by an additional ultracentrifugation step. Different viruses such as Group A rotaviruses, noroviruses (genogroups I and II) and astroviruses were detected with real-time RT-PCR method.
COBISS.SI-ID: 28690905
In this study we have confirmed the validity of a recently published method for rotavirus concentration and detection based on the combination of methacrylate monoliths and realtime reverse transcription-quantitative PCR (RT-qPCR). The method was used to concentrate rotaviruses from different tap water and environmental water samples collected in Slovenia within years 2007 and 2009. The performance of virus concentration using monolithic supports was improved in comparison to the one of tangential ultrafiltration upon application of both methods on a range of environmental samples. Several samples were successfully concentrated on-site after successful adaptation of the method to field requirements.
COBISS.SI-ID: 2291791
In this study experimental results on the effect of electric pulse parameters used for PEF treatment on E. coli present in water samples were shown.
COBISS.SI-ID: 8634708