The NanoSight LM10 with Nanoparticle tracking analysis (NTA) software was evaluated for the quantification of latex particles, and different viruses. The variability was determined by measuring the same sample over several consecutive days and the method’s accuracy was demonstrated by using known concentrations of the subject particles. NTA analysis was also tested to quantify chromatographic fractions of after purification of viruses on a CIM monolithic column. The results demonstrated that nanoparticle tracking analysis is a method for fast estimation of virus concentration in different samples. In addition, it can provide a better insight into the sample status, regarding the level of virus aggregation.
COBISS.SI-ID: 4173432
In this paper the effects of different number of electric pulses, different pulse durations and different pulse amplitudes on inactivation of E. coli are reported. Special attention is given to microsecond pulse duration that affects bacterial cell membrane, nanosecond pulse duration that affects internal structure and combination of both approaches. The synergistic effect of microsecond and nanosecond pulse durations enables the same inactivation rate of bacteria E. coli with smaller energy consumption.
COBISS.SI-ID: 9419604