Mammalian orthoreoviruses (MRVs) are known to cause mild enteric and respiratory infections in humans. They are widespread and infect a broad spectrum of mammals. We report here the first case of a Mammalian orthoreovirus MRV detected in a child with acute gastroenteritis. The whole-genome sequence was obtained with the Ion Torrent next-generation sequencing platform. Prior to sequencing, the stool sample suspension and cell culture supernatant were pretreated with nucleases and/or the convective interaction medium (CIM) monolithic chromatographic method to purify and concentrate the target viral nucleic acid. The same chromatographic conditions as the ones used within the project were selected. It was shown that CIM monolithic chromatography alone is an efficient method for enriching the sample in viral particles before nucleic acid isolation and next-generation sequencing application.
COBISS.SI-ID: 2886735
The high capacity for virus binding of CIM monoliths that allows for virus removal from water samples was also exploited for other appliccations such as purification of viruses. In this achievement CIM monoliths were included in a method for influenza virus purification which could improve vaccine production. This resulted in a patent.
COBISS.SI-ID: 4259192