At the Microbiologia Balkanica, the international conference of experts in microbiology, a part of the results of Jure Zupan's PhD research, which has a direct link to the post doctoral research and represents the basis for further work, was presented. Using the model organism Saccharomyces cerevisiae, for which is known that can cause infections in immunocompromised patients, two methods were established; quantitative agar invasion assay and quantitative profiling of cell wall proteins. The application of these two methods raised the observation that nutrient starvation and temperatures, typical for human fever (37-39 °C), induce strong invasive growth. On the other hand, a repressive effect was observed in the presence of salts, anoxia and some preservatives. The experiment with 64 S. cerevisiae isolates showed a high correlation between invasiveness and virulence of the strains. Analysis of CWPs revealed the presence of up to 20 CWPs for which significant changes in the protein profile expression, relevant to different cultivation temperature, cell morphology (invasive vs. noninvasive growth) and yeast strain, were described.
B.04 Guest lectureCOBISS.SI-ID: 3989880