A fast, reliable, specific and sensitive real-time PCR detection method for three fruit trees infecting phytoplasma was described in this book chapter. The method can be used for the detection of 'Candidatus Phytoplasma mali', 'Ca. P. prunorum', and 'Ca. P. pyri', causal agents of apple proliferation, European stone fruit yellows, and pear decline.
COBISS.SI-ID: 2613839
The paper presents the ten years results of testing many plant and insect samples for the presence of phytoplasma. The stone fruit samples collected during this study and tested for the presence of Ca. P. Prunorum are also presented.
COBISS.SI-ID: 2515279
Sensitivity of field tests, DAS-ELISA, RT-PCR and RT-qPCR were compared for the detection of Plum pox virus (PPV) in various tissues of apricot, peach, plum and damson plum infected with PPV-D, PPV-M or PPV-Rec isolates. We have found that field tests are useful for confirmation of the PPV infection in symptomatic leaves, while in non-symptomatic tissues DAS-ELISA should be combined or replaced by molecular methods.
COBISS.SI-ID: 4448616