The paper reports the results of the analysis of plant and insect samples for phytoplasma infections. The majority of the samples were collected within systematic surveys in the period 2001–2010. Phytoplasmas were found in over 50% of plant samples; in 19 cases mixed phytoplasma infection was identified. Candidatus Phytoplasma mali’, ‘Ca. P. prunorum’ and ‘Ca. P. pyri’, were detected in several fruit-growing areas of Slovenia. The most widespread phytoplasma on grapevine in Slovenia was the stolbur phytoplasma, (‘bois noir’), which was also identified in Convolvulus arvensis and Lycopersicon esculentum. Since 2005, the ‘flavescence dorée’ phytoplasma, has been reported in Slovenian vineyards and also in Clematis vitalba. During the surveys, several known and putative vectors of phytoplasmas were identified.