Coumaphos and amitraz are widely in use for the treatment of honeybee colonies against varosis (Varroa destrucor). Maximal residue levels (MRL) for those compounds in honey is well determined although there are few data for their residue levels in bee brood and beeswax. In light of that, the aim of the study was to determine coumaphos and amitraz levels in honey, bee brood and beeswax after the treatment of bee colonies with those compunds. Coumaphos levels in honey from treated and non-treated bee colonies were found bellow MRL (100 μg/kg) before and after treatment. Coumaphos levels in the brood from treated colonies ranged between 49 and 784 μg/kg. High coumaphos in wax was found in treated and non-treated bee colonies and were ranged between 0.19 and 36 mg/kg. High coumaphos residue accumulation in wax in both treated and non-treated colonies are probably the results of the bee colonies treatments with coumaphos before the reasearch. Contrary, the levels of amitraz in honey, bee brood and beeswax were found bellow the limit of detection (50 μg/kg). To conclude, our study showed the accumulation of coumaphos in brood and beeswax, while for amitraz accumulation was not observed.
F.24 Improvements to existing system-wide, normative and programme solutions, and methods
COBISS.SI-ID: 3916666The aim of the study was to assess the accumulation of coumaphos and amitraz residua in honey, bee brood and beeswax after the treatment of honeybee colonies against varosis. The study was conducted in two apiaries on two different locations. In the first location ten bee colonies were treated with coumaphos and on other location five bee colonies were treated with amitraz. Honey, wax and brood samples were collected before and six weeks after the treatment. Detection of coumaphos and amitraz and its metabolites DPMF, DMF and DMA in honey and brood was performed by HPLC with UV detection, while wax coumaphos and amitraz and its metabolites were determined using GC. Coumaphos level below MRL was found in honey while in the brood from treated colonies coumaphos levels ranged between 49.0 and 784.1 μg/kg. Interestingly, coumaphos in wax was found in treated and non-treated bee colonies. The levels of coumaphos in the wax were high and ranged between 0.19 and 36 mg/kg. High coumaphos residua accumulation in wax in both treated and untreated colonies are probably the results of the bee colonies treatments with coumaphos before the reasearch. However, the levels of amitraz and its metabolites were found bellow limit of detection.
F.24 Improvements to existing system-wide, normative and programme solutions, and methods
COBISS.SI-ID: 3794042Varrosis is a parasitic disease coused by mite Varroa destructor. Varroa supplies itself by bee or bee brood hemolymph. The drugs that act on varroa mites must be used in formations and concentrations that not affect bee colony but they can take effect on parasite. Beside others, coumaphos containing drugs Perizin and ChekMite (Bayer) are used for varrosis treatment in Slovenia. Important role in eradication of varrosis play care for non-contaminated bee products, which are used as a human food, care for environment as well as care for undisturbed bee brood development. Coumaphos use in bee colonies might cause accumulation of its residua or metabolites in honey and other bee products. Coumaphos is stable at low pH, but instable in basic media. In European Union maximal residue limit (MRL) for coumaphos in honey is 100 µg/kg.
F.24 Improvements to existing system-wide, normative and programme solutions, and methods
COBISS.SI-ID: 3883386