A case of Listeria monocytogenes skin infection in a man is presented. Veterinary practitioner developed pustular changes on the skin of arms and hands after assisting with the delivery of a stillborn calf. L. monocytogenes was isolated from the skin lesions and from the bovine placenta. Isolates were serotyped and genotyped PFGE to confirm the suspected transmission of the pathogen from animal to human. To the best of our knowledge, this is the first case of cutaneous listeriosis in which the evidence for zoonotic transmission of L. monocytogenes is supported by genotyping methods.
Since listeriosis, caused by Listeria monocytogenes, is one of the important concerns of public health in Europe related to foodborne zoonoses, an efficient protocol for isolate typing is necessary when performing epidemiological studies. In the present paper, three standardized PFGE protocols available for L. monocytogenes were briefly reviewed. Since observing a poor-quality of ApaI pulsotypes in our laboratory, enzymes from three different manufacturers were compared and modification of the restriction protocol was suggested according to the obtained results.
Listeria monocytogenes and other Listeria species can significantly affect the health of consumers transferring by meat and meat products. Special interest was emphasized due to the possibility of aerogene contamination of meat and products with microorganisms forming bioaerosol. In this paper the presence of Listeria monocytogenes and Listeria spp and in bioaerosol in air of meat processing plants and the importance of selecting the appropriate sampling methods.
The experiment of the synthesis and the coating stability of titanate nanotubes and the outcome experiment of their effects on Listeria reduction in food plants are the results of an interdisciplinary project within the Centre of Excellence NAMASTE. Our experiments in laboratory conditions showed that at least 35% of tested close related nonpathogenic bacteria (L.innocua) were reduced on titanate nanotubes coated glass under the conditions of UV photo influenced excitation (350 nm). The experiment in real conditions that is in poultry slaughterhouse showed more than 2 log10 (99.22%) (in average 40-70%) of test bacteria (L. innocua) reduction on the PE test slides coated by titanate nanotubes induced only by fluorescent light and existent air conditions.
Following the recognition of a mecC MRSA isolate from a patient hospitalized in the northeast region of Slovenia, a national collection of 395 community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates from 2006 to 2013 was screened. An additional six mecC MRSA strains were found and characterized as spa types t843, t9397 and t10009, and multilocus sequence type ST130.