In our research we applied whole-genome sequence typing to characterize a diverse collection of CC398 isolates from animals and humans spanning 19 countries and four continents. Our results strongly suggest that livestock-associated MRSA CC398 originated in humans as MSSA. We assume that the susceptible human strain of S. aureus came into the pig population, acquired a resistance gene and retained athogenicity for humans.
Since Bacillus anthracis is a highly virulent zoonotic pathogen, effective and rapid methods are needed for its detection in different samples. Aerosolized liquid culture of B. anthracis was subjected to four air sampling procedures: most successful detection was with the RCS High Flow sampler (culture-based detection) and when employing the air filter (molecular detection using the SmartHelix DNA extraction kit). Liquid culture was also employed for spiking the homogenized tissue and soil samples for the subsequent DNA extraction using three different commercial kits for each sample type: most successful detection in tissue was with the High Pure PCR Template Preparation Kit and in soil with the PowerSoil DNA Isolation Kit. Results show that B. anthracis can be monitored in different matrices by rapid molecular methods for moderate costs when an appropriate sampling and DNA extraction procedure is employed prior to PCR assay.
The role of hermotolerant campylobacters, the leading cause of bacterial gastroenteritis in humans are described. The primary risk factor associated with intestinal campylobacteriosis is consuming and handling Campylobacter – contaminated poultry meat or meat products. Since all stages of poultry chain from farm-to-fork need to be considered, different professionals and institutions must to be involved in a joint research on the Campylobacter contamination and risk assessment. Cooperation between the Veterinary and Biotechnical Faculty, exist in two current research projects: one on the antibiotic resistance in bacteria of animal origin and the other investigating the problems of Camylobacter contamination of poultry and poultry meat. Since alarming situation was discovered, revealing high resistance of campylobacters against e.g. quinolones, a proper and cautious usage of antibiotics should urgently commence at rearing.