A case of Listeria monocytogenes infection in man is presented. Veterinary practitioner developed pustular changes on the skin of arms and hands after assisting with the delivery of a stillborn calf. This is the first case of cutaneous listeriosis in which the evidence for zoonotic transmission of L. monocytogenesis supported by geno-typing methods.
Three standardized PFGE protocols available for L. monocytogenes were briefly reviewed. Since observing a poor-quality of ApaI pulsotypes in our laboratory, enzymes from three different manufacturers were compared. The obtained pulsotypes showed that restriction digestion with ApaI from New England BioLabs should be complemented with a subsequent overnight incubation of PFGE plugs in TE buffer for better performance.