Fish is an ideal component of a healthy and balanced diet because of its high nutritional value in terms of minerals (P, I, K), unsaturated lipids (oleic acid, linolenic acid and omega 3 series), phospholipids, vitamins (A, D) and proteins of high biological value. They contains also a lot of Se, which is an essential trace element for mammals with a very narrow concentration range between essentiality and toxicity. The aim of this study was to determine total concentration and speciation of Se in four different fish species (creole perch, rainbow trout, brook trout and brown trout) from freshwater lakes in Argentina. Total concentration of Se ranged from 640 to 2470 ng/g on dry matter basis. After enzymatic hydrolysis of sample with Protease XIV, separation and detection of Se species were performed on HPLC using anionic or cationic exchange columns and coupled to ICP MS. In most chromatograms we obtained two peaks; the first peak was unidentified because it could not be attributed to any of the known Se species (Se (IV), Se (VI), Secys2 or SeMetSeCys) while the second peak was identified as selenomethionine (SeMet).
B.03 Paper at an international scientific conference
COBISS.SI-ID: 25158695One of the natural components that may protect against mercury (Hg) toxicity is selenium (Se). Se is an essential trace element for mammals with a very narrow concentration range between essentiality and toxicity [1]. The aim of this study was to determine total concentration and speciation of Se and Hg in fish samples from freshwater lakes in Argentina and what is more to distinguish between distribution of these two elements in soluble and insoluble part of the sample. To measure total concentration of Se, HG-AFS with the chemical and instrumental operating conditions according to Smrkolj and Stibilj was used. After enzymatic hydrolysis of sample with Protease XIV, separation and detection of Se species were performed on HPLC using anionic or cationic exchange columns and coupled to ICP MS. In most chromatograms we obtained two peaks; the first peak was unidentified, while the second peak was identified as selenomethionine (SeMet). Total concentration of Hg and MeHg and their partitioning between soluble and insoluble part after enzymatic hydrolysis were also obtained.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 24917543