The aim of this study was to determine total concentration of selenium, its species and seasonal variations in marine mussel Mytilus Galloprovincialis from different locations. They were collected at three different locations along the north eastern coast of Adriatic Sea during 3 year period from 2009 to 2011. Total Se concentrations in mussels varied between seasons; in spring concentrations of Se were much higher than in autumn, around 5 µg Se/g and 2 µg Se/g, respectively regardless of rearing conditions. After enzymatic hydrolysis of sample with Protease XIV, separation of soluble Se species were performed on HPLC using anionic or cationic exchange columns coupled to detector ICP-MS where SeMet and another unknown compound were determined.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 25926695Marine organisms such as mussels and fish take up polonium (Po) and selenium (Se), and can accumulate them into cellular components. Po-210 is highly radiotoxic due to its high specific activity of 1.7 x E+14 Bq/g, while selenium is an essential trace element for humans and animals, since it forms part of several enzymes. The aim of our study was to investigate distribution of Po-210 and Se between soluble and insoluble fraction of mussel samples (Mytilus galloprovincialis), fresh and cooked, obtained after water and enzymatic extraction. After water extraction from 31 to 37 % of Se in mussel samples and approximately 15 % of Se in cooked mussel samples was present in soluble fraction. Contrary, up to 5 % of Po-210 remained in soluble fraction of both fresh and cooked mussels after water extraction, which indicates that polonium is not bound to water soluble proteins. After enzymatic extraction with protease XIV from 58 to 72 % of Se from fresh mussels and 57 % of Se from cooked mussels was found in soluble fraction. Moreover, the fraction of soluble Se after enzymatic extraction was much higher than after aqueous extraction. There were approximately 30 % more soluble Po-210 and Se found in supernatant after enzymatic hydrolysis as in case of water leaching. Therefore, around 70 % of Po-210 contained in the mussels remained insoluble, and thus more biologically unavailable, while around 70 % of Se was soluble and more readily bioavailable.
B.04 Guest lecture
COBISS.SI-ID: 26554151