In our study we included two main fish species that represent the most commonly consumed fresh fishes in Slovenia; trout (Oncorhynchus mykiss) and seabass (Dicentrarchus labrax) both from different locations (Slovenia, Italy, Greece) and environments (fish grown up in fish farms and wild fishes). Total Se concentrations were measured by HG-AFS [4], speciation in soluble fraction after enzymatic extraction was performed by HPLC-ICP-MS, while other elements were determined after microwave digestion by ICP-MS. The main Se species were selenomethionine and selenocystine in soluble fraction, between 80-100%, exception was the wild seabass soluble fraction, where only 73 % of soluble Se was identified. Content of toxic elements, such as cadmium (Cd) and lead (Pb) were under the detection limit (3 and 23 ng/g respectively) regardless fish species. Se concentrations were more variable; in trout concentrations ranged from 120 to 211 ng/g on wet mass basis while in seabass Se concentrations varied regarding fish origin and breeding method. In seabass, which were grown up in fish farms Se concentrations were in the same range as in trout, whereas seabass caught from natural environment have significantly higher concentrations around 400 ng/g.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 26908711The highest Zn levels were found in Anchovies and sardines from Italy and Croatia (19.2 ± 1.7 µg/g fw), followed by squids (from 12.0 to 18.8 µg/g fw), whereas the lowest amounts were found in panga fillets (2.8 ± 1.9 µg/g fw). According to Reference values for nutrient intake set by DACH for Zn of 10 mg/day, values obtained in this research represent from 2 to 41 % of recommended daily intake by eating 150 g portion of fish or squids. The highest concentrations of Fe ere also found in Anchovies and sardines from Italy and Croatia (29.6 ± 12.0 µg/g fw) and rainbow trout from Italy and Bosnia (15.6 ± 14.5 µg/g fw), whereas in other samples the concentrations were below 10 µg/g fw. As for the Zn, the recommended daily intake (RDI) for Fe is 10 mg/day and samples in this work meet from 1 to 62 % of RDI, where the lowest amount is achieved by eating panga fillet and the highest by consumption of anchovies or sardines. The mean concentrations of Cu did not differ significantly between different fish species, except for the Patagonian squids, where the level was 10.1 ± 1.4 µg/g fw. Our obtained values are in good agreement with the literature data (Romeo et al., 1999, Tüzen, 2003, Usero et al., 2003, Henry et al., 2004). The determined values achieved up to 26 % of RDI for Cu (1 - 1.5 mg/day), expect for the Patagonian squids, where the values were above RDI, 110 %. According to the Joint Committee of FAO/WHO toxic limit for Cu of 30 mg/kg/person/week the amount of Cu in these squids were still below the limit (FAO/WHO, 2004). Selenium (Se) concentrations did not vary a lot between different groups of fish, from 0.1 to 0.7 µg/g fw. Our obtained data are in a good agreement according to the literature, where Se concentrations ranged from 0.1 to 1.2 µg/g fw (Miklavčič et al., 2011, Cabañero et al., 2004, Ciardullo et al., 2008).Concentration levels for Cd and Pb in this present study were mostly below the limits of the detection obtained on the ICP MS, 0.003 and 0.023 µg/g, respectively. However, the highest amount of Cd was found in squids from Patagonia, 0.7 ± 0.1 µg/g fw, while for Pb the highest concentrations were found in the group trout from Italy and Bosnia 0.1 ± 0.06 µg/g fw.
B.06 Other
COBISS.SI-ID: 27574567