The analysis of biological samples in frozen-hydrated state with micro-PIXE technique at Jožef Stefan Institute (JSI) nuclear microprobe has matured to a point that enables us to measure and examine frozen tissue samples routinely as a standard research method. Cryotome-cut slice of frozen-hydrated biological sample is mounted between two thin foils and positioned on the sample holder. The temperature of the cold stage in the measuring chamber is kept below 130 K throughout the insertion of the samples and the proton beam exposure. Matrix composition of frozen-hydrated tissue is consisted mostly of ice. Sample deterioration during proton beam exposure is monitored during the experiment, as both Elastic Backscattering Spectrometry (EBS) and Scanning Transmission Ion Microscopy (STIM) in on–off axis geometry are recorded together with the events in two PIXE detectors and backscattered ions from the chopper in a single list-mode file.
F.12 Improvements to an existing serviceCOBISS.SI-ID: 3343695
We coorganised International congres in Reka (Cro) with more than 200 participants from 15 countries. Plenary and short lectures and posters presented main topics from biodiversity, taxonomy evolution, conservation biology, growth and development as well as interactions of plants. In addition scientific excursions were organized.
B.01 Organiser of a scientific meeting
Acta Biologica Slovenica (former Biološki vestnik) is Slovene scientific journal, which has been publishing for more than half of the century. It publishes peer reviewed papers from the field of biology twice a year. Biological library distributes the journal to 188 institutions in Slovenia and worldwide, receiving other scientific journals for exchange.
C.05 Editorial board of a national magazine
The textbook emphasizes the holistic approach to ecosystems, which had been introduced by E-P. Odum. Holistic approach is the basis for effective management of natural resources and ecosystems and for long-term preservation of ecosystem services. The textbook was written for students who take the classes in Ecology as a supplement to other scientific fields or as a basis for further deepening of knowledge.
B.06 OtherCOBISS.SI-ID: 281166080
The contribution discusses the relationships between reflectance spectra obtained by field spectroscopy and properties of the leaves of the species that form a stand and the relation between reflectance spectra and stand characteristics. We studied monospecific and mixed stands that thrive in habitats along a hydrological gradient in the intermittent Lake Cerknica. The reflectance spectra differed significantly at the stand and leaf levels; however, although the shape of the reflectance spectra of a monospecific stand with Phalaris arundinacea was similar to the shape of the leaf spectra, this was not the case for mixed stands. The leaf morphological and biochemical properties that explain most of the variability of the spectra differed for graminoids and different dicotyledons. This study shows that based on the reflectance spectra, the species properties for monospecific stands can be deduced, while for mixed stands, such deductions can be misleading.
F.02 Acquisition of new scientific knowledgeCOBISS.SI-ID: 3223119