Genetic differentiation of 167 Phaseolus vulgaris L. accessions originating from five South east European countries was performed using 13 SSR markers. Analysis included 21 accessions from Bosnia and Herzegovina, 15 accessions from Croatia, 73 accessions from Macedonia, 38 accessions from Serbia and 20 accessions from Slovenia. Calculated mean number of alleles per locus was 9.8. The average polymorphic information content over all loci reached value of 0.710 where the most informative locus was GATS91 (0.926). These results showed that selected set of SSR markers are highly informative and applicable for studies of genetic diversity within germplasm collected in the five South east European countries, which was also confirmed with the calculated value of probability of identity (1.831x1014). Analysis of molecular variance showed only 8 % genetic variability among accessions of P. vulgaris from different countries which indicates their common origin. Based on genetic distance, UPGMA clustering method and Bayesian clustering analysis, the accessions from five countries are generally divided into four genetic groups.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 4192872In recent years seminatural grassland has gained a multifunctional role in the environment. In addition to providing forage for farm animals, many other functions of seminatural grassland, including biodiversity, are even so important. From this point of view, grasslands of high natural value are of high significance. Throughout the history, biodiversity has been maintained primarily by the extensive grazing and harvesting hay during seed maturation. With the intensification of grassland management in the 20th century and the use of commercial seed mixtures on the seminatural grasslands, biodiversity of the sward in general began to decline. We have recently become aware of the negative consequences of declining biodiversity and begun to develop measures to stop this process. Among these measures, establishment and restoration of seminatural grassland with high natural value play an important role. Seed material for the establishment and restoration of such sward can be obtained only from species rich seminatural grassland. In recent years a variety of methods have been developed all over the world for seed production and sowing on seminatural grasslands, allowing successful reestablishment of original biodiversity, adapted to local growing conditions.
F.17 Transfer of existing technologies, know-how, methods and procedures into practice
COBISS.SI-ID: 4158568Sampling is the first critical step that affects the reliability of any analytical testing, since it is always a source of error. A good and reliable sampling procedure, which is of crucial importance in GMO traceability, should minimize this unavoidable error whilst maximizing the representative nature of the sample. In the chapter entitled ‘GMO sampling strategies in food and feed chain’ a sub-chapter is dedicated to development of sampling schemes to ensure co-existence in the field. First, visualization of spatial variability of outcrossing rate using geostatistical tools is presented, followed by data mining and modelling of the spatial variability of the outcrossing rate. Distribution of outcrossing rate in the field is presented for different field types. Development of sampling approaches for determination of adventitious presence of GMO in the field before harvest is described in details.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 2673743Featuring relatively short entries on genetics topics written by experts in that topic, Brenner’s Encyclopedia of Genetics provides an effective way to quickly learn about any aspect of genetics, from Abortive Transduction to Zygotes. Adding to its utility, the work provides short entries that briefly define key terms, and a guide to additional reading and relevant websites for further study. Many of the entries include figures to explain difficult concepts. Chapter discusses role Morpho-agronomic traits in the plant breeding process and in the development of improved varieties that made a major contribution to the increased productivity and quality of plants used for their food, feed, fiber, or esthetic value.
F.30 Professional assessment of the situation
COBISS.SI-ID: 4246120The book in it's 13 chapters discusses specific concepts related to potato culture, it's agrobiodiversity and conservation, not solely in Montenegro but as well worldwide. Second part of the book is dedicated to a catalogue of the most important potato accessions preserved in the Gene Bank of Montenegro.
F.01 Acquisition of new practical knowledge, information and skills
COBISS.SI-ID: 4298856