Close relationsip between B. oleracea and S. arvensis is reflected in a high level of morphological, genetic and karyotypic similarity. Consequently, it is difficult to distinguish these two species, especially when analyzing seed purity in seed testing laboratories. The problem lies primarily in seed identification, where seeds of S. arvensis represent impurities in B. oleracea seed lots. The method to distinguish between these two species according to the seed coat morphology, using binocular, is not reliable. To distinguish between B. oleracea and S. arvensis species on molecular level, we developed a fast and reliable SSR method using genome-specific markers. We tested 15 highly informative SSR markers, originating from different Brassica species. Two markers (Na12-C08 and Ra2-E12) were proven to be genome-specific due to their successful amplification in B. oleracea only, but not in any of the S. arvensis samples. The third marker (Ra2-A01) was amplified in all samples, but in different allele forms that distinguished B. oleracea and S. arvensis genomes. This locus could also be applied for intra-species differentiation within S. arvensis genome. The use of genome-specific markers (Na12-C08, Ra2-E12 and Ra2-A01) in seed testing laboratories would provide a fast, reliable and cost - effective tool for qualitative genetic screening and identification of S. arvensis seeds as impurities in B. oleracea seed lots or vice versa.
F.17 Transfer of existing technologies, know-how, methods and procedures into practice
COBISS.SI-ID: 4523624In the invited lecture the results of studies of drought stress response in common bean in the last ten years were presented with Slovene and partners abroad. These included studies on morphological, physiological, transcriptome and proteome level. In the last years the research was focused towards the studies of drought stress response in two cultivars differing in the responese to drought, Tiber (tolerant) and Starozagorski čern (susceptible) using proteomics. The two cultivars were used for generation of 82 recombinant inbred lines of F8 generation. A genetic map for QTL analysis was developed. The knowledge of mechanisms of drought stress response is important for identification of traits used in common bean breeding for drought tolerance.
B.04 Guest lecture
COBISS.SI-ID: 4522856Our work includes also the transfer of our knowledge and our results to other researchers and end users. We participate at different national and international conferences and organize education courses for different authorities (inspection service, extension service, growers…). We also prepare information on important pests and disease and publish them on our web page (COBISS.SI-ID 4532072, 4523880, 4612200, 4609640, 4409960, 4441192 )
F.18 Transfer of new know-how to direct users (seminars, fora, conferences)
COBISS.SI-ID: 4532072The intense five-day course on Agricultural Omics was opened to a wide range of top scientific institutions, including CropSustaIn project partners and other agricultural institutes already collaborating with EMBL-EBI. The trainers were specialists in their fields from EMBL-EBI and collaborating institutes. This course provided an overview of data resources, tools and analysis pipelines for ‘omics datasets within the agricultural sciences. The course was delivered using a mixture of lectures, practical computer sessions and discussions based on agricultural case studies and public domain data. Additionally there was opportunity for attendees to review the key opportunities and challenges within the field. Altogether thirty (30) participants attended the course. Ten (10) participants were from the Agricultural Institute of Slovenia, tree (3) participants were from other Slovene institutions, while the other seventeen (17) participants were representatives of agriculture related research institutions and universities from different countries. The substantial number of trainers (13) delivered high quality lectures and provided individual attention to help to the participants to get the most from practical sessions for a personalized learning experience. The trainers also contributed to the networking opportunities, interacting with participants to discuss particular topics.
B.01 Organiser of a scientific meeting
COBISS.SI-ID: 4648808The disease crown gall, which causes damage on perennial agricultural crops, is economically important in many countries. We therefore explored the presence of Agrobacterium spp. in a variety of agricultural soils where fruit trees are grown. Crown gall-affected plants were not observed on any of the sampling sites. Members of Agrobacterium spp. biovar 1 were isolated from 63 of the 72 soil samples (88%). All isolates of this Agrobacterium species complex were determined to be non-pathogenic by biotests and were assigned to genomic species based on a recA allele sequence analysis. Fourty-three isolates were allocated into genomic species G1 and 26 isolates into G4. Among both genomic species, new alleles of the recA gene were recognized: seven new alleles in G1 and four in G4. Different colony morphologies were observed between strains of G1 and G4 on KB medium.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 4474472