A proteomic approach was used to identify droughtresponsive proteins in leaves of two cultivars of common bean (Phaseolus vulgaris L.) differing in their response to drought, more sensitive Starozagorski čern and Tiber. 2D-DIGE was used to compare differences in protein abundance between control and stressed plants. Fiftyeight proteins whose abundance changed significantly were identified by LC-MS/MS in Tiber and 64 in Starozagorski čern. The majority of identified proteins were classified into functional categories that include energy metabolism, photosynthesis, ATP interconversion, protein synthesis and proteolysis, stress and defence related proteins. Details of the function of the identified proteins and their abundance profiles in Tiber and Starozagorski are discussed. Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways and molecular functions affected by drought stress.
COBISS.SI-ID: 3924072
Protein breakdown by proteases is basic to the plant response to abiotic stresses such as drought. A large number of genes encoding proteases or putative proteases exist in plants. Only a few of those involved in the response to drought have been characterized, and their regulation is poorly understood. We have identified two new subtilases from leaves of Phaseolus vulgaris L. cultivar Zorin, PvSLP1 and PvSLP2. PvSLP1 was identified at the gene level, using primers based on the gene sequence of the putative drought induced serine protease from Arachis hypogaea L. In P. vulgaris, expression of the PvSLP1 transcript did not change on water withdrawal. PvSLP2 was isolated and characterized at the protein level, together with complete gene and cDNA sequences. The deduced amino acid sequences of both PvSLP1 and PvSLP2 are characteristic of plant subtilases of the S8 family of clan SB. PvSLP2 shows 33% sequence identity to PvSLP1. Expression of the PvSLP2 transcript did not change on withdrawal of water, but its proteolytic activity in leaves increased, depending on the age and position of the leaf. In addition, the level of activity in senescent leaves of well watered plants was higher than in mature or young leaves. These results, together with the fact that PvSLP2 cleaves peptide bonds following an Arg residue, point to regulation of PvSLP2 subtilase activity at translational and/or post-translational levels and suggest a specific role in the response to drought and senescence.
COBISS.SI-ID: 26305831
Common bean (Phaseolus vulgaris L.) was introduced in Europe from both Mesoamerican and Andean centers of origin and has been cultivated in central Europe for centuries. The first objective of this study was to evaluate genetic diversity and the population structure of 167 accessions divided into four groups according to geographical origin (Slovenia and Austria) and time periods (historical and present) using 14 simple sequence repeat (SSR) markers. The second objective was to improve our understanding of the pathways of dissemination and the evolution of this species in central Europe. Great allelic polymorphism was detected in all four groups of examined accessions, indicating that Slovene and Austrian bean germplasm in the past possessed significant variation that has been well preserved until today. In factorial correspondence analysis, accessions from different groups clustered together indicating potential gene flow between countries. The uncovered diversity corresponded very well to the two recognized gene pools (Andean and Mesoamerican). The majority of accessions in every single group belonged to the Andean gene pool. Strong predominance of Andean genotypes classifies Slovenia among other Mediterranean countries, like Spain and Italy. The latter appears as a most probable source of first beans in Slovenia and Austria. We assume that in the beginning of the previous century after very tight relationships between Slovenia and Austria loosened, introgression of genotypes from western and northern European countries took place in Austria, which resulted in a very high proportion of Mesoamerican genotypes that we found in the present Austrian germplasm (44%). Several putative hybrids between the Andean and Mesoamerican gene pools were detected in this study.
COBISS.SI-ID: 4208744
According to International Code of Nomenclature for algae, fungi, and plants, new rules apply since 1st January 2013 for the naming of pleomorphic fungi. As a result of an analysis, the generic naming of important plant pathogenic fungi and biological control agents was discussed and the use of Clonostachys over Bionectria, Trichoderma over Hypocrea, Fusarium over Gibberella, and Neonectria over Cylindrocarpon (etc.) recommended.
COBISS.SI-ID: 4289896
Analysis of gene flow between Brassica napus L. and its sexually compatible relatives that could be found in the wild in Slovenia was performed by microsatellite analysis using fifteen selected primer pairs. Genotypes included in the study were obtained from the field survey of sexually compatible relatives of B. napus in natural habitats around Slovenia and from reference collections. Two different wild species of all the presented sexually compatible relatives of B. napus were found in Slovenia, B. rapa and Sinapis arvensis. The reference genotypes included varieties and wild forms from internal collections as marketable seeds or from gene banks. Reference genotypes were represented by the following species and subspecies: B. napus ssp. napobrassica, B. napus ssp. napus, B. nigra, B. oleracea, B. rapa ssp. oleifera, Diplotaxis muralis; D. tenuifolia, Raphanus raphanistrum, R. sativus, R. sativus var. oleiformis, Rapistrum rugosum, S. alba and S. arvensis. Estimation of gene flow described by average number of migrants was 0.72 followed by 0.20 migrants. We found four highly divergent and polymorphic loci (Na12-C08, Na10-A08, Ni3-G04b and BRMS-050) at statistically significant level (p(0.05) of gene flow detected. The 30.7 % out-crossing rate of B. rapa and S. arvensis populations from filed survey represents the potential for spontaneous inter-and intra-specific gene flow under Slovenian production area.
COBISS.SI-ID: 4298088