The present invention describes a device for in-vitro controlled release of active ingredients. The essential invention of this kind of devices` embodiment is that does not use intermediate barrier (membrane) between the wound dressing, with integrated active substance and receiving media, to simulate the actual conditions in the wound. In present invention the wound dressing on a holder comes into direct contact with the liquid in the receptacle; therefore, the release of active compound is activated. The advantage of such devices` embodiment is that it simulates the release of active substance in chronic wounds and wounds with large exudate (secretions).
F.33 Slovenian patent
COBISS.SI-ID: 19563542The invention relates to a method for the preparation of a pH-indicator electrospun nanofibres based on polysaccharides incorporated with pH-sensitive dye for fast and easy evaluation of the changes in pH of the exudate (secretions) in the wound. Essential invention proposed the method of production of nanofibres by i) preparing a solution of cellulose acetate suitable viscosity rather toxic, highly volatile / flammable solvent acetic acid is used, and ii) using a such halokromno dye which is easy to dissolve in the spinning solution, and does not affect the morphology of the fibers and at the same time fast, important and irreplaceable changes color depending on the pH of the medium.
F.33 Slovenian patent
COBISS.SI-ID: 19333654Diabetes has an growing negative impact on healthcare systems around the world with an annually increasing number of patients around 0,5%. Amongst the more frequent complications of diabetes with significant implications on the patient quality of life, is the diabetic foot. Since related consequences can be severe, different preventive measures have been considered over the last years to either improve early detection or to prolong the time until the diabetic foot can arise. Over the past decade, a lot of work has been conducted to improve efficiency of antimicrobial agents, their selectivity, and even specificity. Yet there are still no products available, dealing with prevention or disturbance of pathologic bacteria growth by using the body’s natural allies that form the healthy skin microbiome. To the best of our knowledge, using the promotion of “healthy bacteria” growth to hinder habitation by pathologic bacteria, is something new even in research. Based on the mentioned, our main goal will be the preparation of novel textile materials for prophylactic treatment of the diabetic foot. A specially designed approach will be used for functionalization of commercial fibers, where we will use methods for increasing the electrostatic interactions between bacteria and fibers. Novel textile materials will be developed also by using the environmentally friendly technology electrospinning, where careful design of nanofibers with tuned size and chemistry, will enable the incorporation of beneficial microbiota and add to the function of the final product.
F.18 Transfer of new know-how to direct users (seminars, fora, conferences)
COBISS.SI-ID: 19808534The aim of the present study was to prepare a polysaccharide based nanofibrous sensor for detection of pH change in the wound environment. In order to prepare cellulose acetate (CA) nanofibers, acetic acid was used as a solvent, and fabrication of fibers was performed on the needle-less electrospinning apparatus. Long uniform CA nanofibers, with diameters ranging from 250 to 300 nm, were electrospun from 15wt% CA and 85% acetic acid, with addition of halochromic dye (Bromocrezol Green). The addition of Bromocrezol Green in the spinning formulation did not affect the fiber formation. Prepared nanofibrous sensors were characterized using CIE color space analysis in order to evaluate the color due to pH change. Nanofibrous sensors exhibit yellow color when exposed to pH4 and lower, simulating the wound environment beneficial to the wound healing, and blue color when exposed to pH9 and higher, simulating the environment that hampers wound healing (chronic, infected wounds).
B.03 Paper at an international scientific conference
COBISS.SI-ID: 18953494The patent describes the process for impregnation of porous carriers with bacterial cells and the process for restraining the cells within the pores of these porous carriers. The porous carriers have the cells immobilized using linear polymers, which allow the crosslinking of cells within the matrix or their electrostatic binding, and are useful for the implementation in biotechnological processes for the purpose of obtaining active compounds from immobilized cell mass or the degradation of a target compound being introduced into the system. The main advantage of such carriers is that the cells are artificially attached within the pores of the carriers, meaning they are protected from external factors and their release from the carriers is limited. Consequently, the contamination of the final product with the cells is reduced. The porous carriers, prepared as described in the patent, can be used in continuous or batch biotechnological processes.
F.33 Slovenian patent
COBISS.SI-ID: 37774085