Wound treatment underwent a complex evolution towards smarter and more efficient therapeutic and diagnostic interventions, while putting extreme weight onto the patient safety. The most important factors influencing wound healing are the maintenance of proper moisture in and above the wound, assuring a bacteria free environment, and pain reduction. We address these two topics, proper hydrophilicity to maintain desired moisture and pain reduction for effective healing. Regarding the first point, we found that plasma treatment can provide simple and effective hydrophilicity tunings, using one or two stages. Using ammonium based plasma as the source, we can achieve a significantly increased material hydrophilicity, as well as add some antimicrobial activity simultaneously. We also addressed pain reduction for effective healing, focusing on model thin film based systems to lower the development costs, as well as to study the potential of different materials for effective drug release control. The thin film preparation methodology proved to be highly comparable to real systems. To prepare best possible delivery solutions for different wound types, we varied the use of base materials, method of drug incorporation and the drug type, and performed in vitro studies.
B.04 Guest lecture
COBISS.SI-ID: 17315862Efficient wound treatment is not possible without thorough knowledge about wound physiology. The first lesson in wound healing is that optimal healing requires a moist and bacteria free environment. Although achievement of both is not that difficult, we often overlook the influence of moisture on the mechanical properties of the host materials and the function of active ingredients. Additionally is an efficient antimicrobial activity often achieved at the cost of the dressings’ overall safety. Therefore, we present in this study the preparation of novel cellulose-based materials exhibiting a safe antimicrobial activity, while simultaneously assuring a desired moist wound environment. Irreversibly bound silver nanoparticles and a plasma treated surface layer served for the first and special plasma treatment procedures resulted in the second. Novel cellulose-based materials, prepared in such a way, were shown to be safe for use and a preliminary analysis also proved their efficiency.
B.04 Guest lecture
COBISS.SI-ID: 512281400The invention is based on the preparation of solid, liquid-liquid, solid-liquid and semisolid media, which enables cultivation of two bacteria in the same space as a co-culture. By using different dyes based on the pH and redox sensitivity it is possible to measure growth rate and effects of one bacteria to another in this co-culture. The procedure is adapted to be used in a 96 well format and enables growth at different temperatures in the gradient thermo block in a PCR machine.
F.33 Slovenian patent
COBISS.SI-ID: 30515673