A novel two-layered pain relieving wound dressing was prepared from a combination of biocompatible polymers carboxymethylcellulose and polyethyleneoxide, and two types of pain relieving drugs, the non-steroid anti-inflammatory diclofenac and the local anesthetic lidocaine. To achieve the two-layered structure, electrospinning and impregnation of a commercially available wound dressing Aquacel, were used for preparation of respective layers. The electrospun nanofibers have been shown to possess similar features as found in the extracellular matrix (ECM), an important component of the skin. This characteristic could significantly contribute to the efficiency of wound healing. The second layer is based on Aquacel, an important wound dressing in modern wound care. Since pain can drastically lower the wound healing process, as well as it is known to decrease the overall quality of patient life, pain relieving drugs are very interesting for wound care applications. For efficient pain reduction, two types of drugs were used. When combined, these can cover different types of wound related pain (due to the cause and treatment), and hence additionally aid the wound healing process. The combined features of the incorporated pain relieving drugs and the mentioned materials are therefore very interesting for future studies towards clinical testing of possible prototype products.
COBISS.SI-ID: 19019798
An elegant way for the controlled production of either cellulose fibers or hollow spheres from trimethylsilyl cellulose (TMSC) via needle-less electrospinning is reported. The approach takes advantage of different degrees of molecular entanglements caused by differences in origin and molecular weight of the cellulosic source.
COBISS.SI-ID: 18865686
Considering the increasing resistance of numerous bacteria to antibiotics, a novel wound dressing material was developed with naturally acquired olive leaf extract, which shows not only good antimicrobial activity, but also very good antioxidant activity. Environmental friendly procedure, electrospinning, was used for the first time, to prepare polysaccharide nanofibrous mat with incorporated olive leaf extract, with the unique property of releasing the active phenolic components in a prolonged manner of 24 hours. Developed electrospun mats were characterized using scanning electron microscopy (SEM), high performance liquid chromatography (HPLC), UV/VIS spectroscopy for determination of free radical scavenging activity by DPPH, antimicrobial testing and release kinetics. Antimicrobial tests have shown that electrospun mat with olive leaf extract achieve reduction towards tested microorganisms: Staphylococcus aureus (G+), Escherichia coli (G-), Enterococcus faecalis (G+), and Pseudomonas aeruginosa (G-); while the high antioxidant activity of olive leaf extract is preserved during the electrospinning procedure. Release of olive leaf extract from electrospun mat was mathematically modelled, and the release kinetics results follow the Korsmayer-Peppas model indicating the release ability due to erosion of polysaccharide nanofiber mat.
COBISS.SI-ID: 19351062
The present invention relates to a carrier of silica (QCM - Quartz Crystal microbalance) crystal, which can easily be mounted or removed from electrospinning device. Essential invention of designed bracket is that it allows in-situ formation of nanofibres formed on a quartz crystal, used for further analysis on quartz microbalance (evaluation of weight and thickness of electrpspun nanofibrous mat, the adsorptive properties of nanofibres). The holder is designed so that it does not affect the operation of electrospinning device, nor the characteristics of quartz crystal and formation of electrospun fibers. With the use of invented holder we can electrospun nanofibers, on the active measuring surface of the quartz crystal, from a variety of synthetic and natural polymers with or without additives.
COBISS.SI-ID: 19105814
The aim of the present study was to prepare a polysaccharide based nanofibrous sensor for detection of pH change in the wound environment. In order to prepare cellulose acetate (CA) nanofibers, acetic acid was used as a solvent, and fabrication of fibers was performed on the needle-less electrospinning apparatus. Long uniform CA nanofibers, with diameters ranging from 250 to 300 nm, were electrospun from 15wt% CA and 85% acetic acid, with addition of halochromic dye (Bromocrezol Green). The addition of Bromocrezol Green in the spinning formulation did not affect the fiber formation. Prepared nanofibrous sensors were characterized using CIE color space analysis in order to evaluate the color due to pH change. Nanofibrous sensors exhibit yellow color when exposed to pH4 and lower, simulating the wound environment beneficial to the wound healing, and blue color when exposed to pH9 and higher, simulating the environment that hampers wound healing (chronic, infected wounds).
COBISS.SI-ID: 20343062