This paper describes establishment of international collection of animal C. difficile strains, their characterization and distribution of ribotypes across animal species and countries.
Following the recognition of a mecC MRSA isolate from a patient hospitalized in the northeast region of Slovenia, a national collection of 395 community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates from 2006 to 2013 was screened. An additional six mecC MRSA strains were found and characterized as spa types t843, t9397 and t10009, and multilocus sequence type ST130.
The results of this study indicate that young domestic small ruminants, aged up to 16 days, may also be the reservoir of C. difficile and are colonized with similiar PCR-ribotypes as other animals and humans. PCR-ribotype 014/020 found in goat is the most prevalent in Slovenia and was also isolated from humans, poultry, calves, dog and the environment. With PFGE, three pulsotypes were indistinguishable from restriction patterns in our PFGE database of animal isolates.