This paper describes susceptibility patterns, virulence gene profile, clonality and epidemiology of community associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in Slovenia. Among 151 isolates, the mecA gene was detected in 150 isolates, while the mecC gene only in 1 isolate. The MRSA isolates were classified to 19 different clones. The most prevalent sequence types were ST5 (26.4%), ST45 (25.2%), ST22 (10.6%), ST398 (9.9%), ST8 (5.9%), ST7 (4.6%), ST1 (3.9%), ST152/377 (3.3%), ST228 (2.6%) and ST2883 (1.3%). The ST6, ST9, ST30, ST72, ST88, ST111, ST130, ST225 and ST772 were identified sporadically. The Panton-Valentine leukocidin (PVL) gene was detected in 13 (8.6%) isolates that belonged to ST5, ST7, ST8, ST22, ST72, ST88, ST 152/377 and ST772.
This paper describes molecular characterization of methicillin-resistant Staphylococcus aureus, ST398 (LA-MRSA), from human samples. During the year 2010, we found 15 (9.9%) MRSA isolates, that belonged to a spa type known to be associated with the clone ST398 respectively. 12 isolates belonged to spa type t011, 2 isolates to t034 and 1 isolate to t108. All MRSA isolates were resistant to tetracycline and penicillin. Some of them were also resistant to erythromycin and clindamycin. Most of the LA-MRSA ST398 were isolated from screening specimens of patients from Murska Sobota and Maribor, which are the most important agricultural regions with intensive livestock breeding. Evidence of the presence of LA-MRSA in humans requires a close cooperation of human and veterinary microbiologists. Our goal is to find the epidemiological relation between human and animal hosts, to obtain information on the phenotypic and genotypic characteristics and monitor infections caused by LA - MRSA strains.
This paper describes the first mecC positive MRSA isolate that was isolated from a patient hospitalized in the northeast region of Slovenia. An additional six mecC MRSA strains were found among a national collection of 395 community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates from 2006 to 2013. MecC MRSA isolates belonged to spa types t843, t9397 and t10009, and multilocus sequence type ST130.