In thesis the optimization of electric pulse parameters for protein extraction from E. coli bacteria was described. We found that extraction of proteins by means of electroporation is more efficient using longer pulses with lower amplitude, where the ratio between concentration of extracted proteins and bacterial survival is most favourable.
D.10 Educational activities
COBISS.SI-ID: 10655060In the article electroporation and its widely use in different applications is presented. Among others also extraction by means of electroporation in food industry is described.
F.18 Transfer of new know-how to direct users (seminars, fora, conferences)
COBISS.SI-ID: 10504276