We described a relatively efficient and facile procedure for extraction of proteins from E. coli — extraction by means of membrane electroporation, caused by exposure of cells to electric pulses. Different parameters (temperature, bacterial growth phase) were studied in order to obtain maximum protein concentration and in parallel bacterial inactivation was studied. Based on our results we conclude that temperature is a key element for bacterial membrane to stay in a permeabilized state, so more proteins flow out of bacteria into surrounding media.
COBISS.SI-ID: 11506516
Our goal was to study different electric pulse parameters in order to extract as much proteins as possible from E. coli bacteria, while preserving bacterial viability. Our results show that by increasing electric field strength the concentration of extracted proteins increases and viability reduces. While at longer pulses (with lower electric field strength) we obtained more proteins, but bacterial viability was not as much affected. We can conclude that the most promising pulse protocol for protein extraction by means of electroporation based on our experience would be longer pulses with lower pulse amplitude assuring high protein yield and low effect on bacterial viability.
COBISS.SI-ID: 11153492
In this study we tested a prototype pulse generator that enables continuous pulsed electric field treatment for Escherichia coli inactivation and microalgae lipid extraction.
COBISS.SI-ID: 10505556
We outline electroporation as a phenomenon and then proceed to applications where we among others described recent advances in applying electroporation for inactivation of microorganisms and extraction of biomolecules. Article was published in prestige journal Trends in Biotechnology with high impact factor (5-year impact factor was 13,382). This work was awarded by ARRS as Excellent in Science in 2016.
COBISS.SI-ID: 11070548
In chapter we describe electroporation as a method for extracting technologically relevant molecules (proteins, plasmid DNA, lipids) from microorganisms (bacterial and yeast cells). At the end main parameters affecting the yield of extracted molecules are summarized and main challenges of extraction by means of electroporation of molecules from microorganisms are presented.
COBISS.SI-ID: 11714644