We developed a vector design strategy for plant synthetic biology. The genetic language was formalized and implemented as a grammar in GenoCAD, a Computer-Aided Design software for synthetic biology. It includes a library of plant biological parts organized in structural categories and a set of rules describing how to assemble these parts into large constructs. Rules developed here are organized and divided into three main subsections according to the aim of the final construct: protein localization studies, promoter analysis and protein-protein interaction experiments. The GenoCAD plant grammar guides the user through the design while allowing users to customize vectors according to their needs. Therefore, the plant grammar implemented in GenoCAD will help plant biologists take advantage of methods from synthetic biology to design expression vectors supporting their research projects.
COBISS.SI-ID: 3528271
A comprehensive analysis of the dynamic changes in primary metabolism of potato was performed, which included whole transcriptome analysis, nontargeted proteomics and photosynthetic activity measurements in the plants of cv. Désirée and the transgenic NahG- Désirée. We show that while the dynamics of responses at the transcriptional level are extensive and bimodal, this is only partially translated to the protein level, and to the final functional outcome.
COBISS.SI-ID: 3614031