Artificial plant inoculations methods present important tool in plant virology, and could be used in different studies such as resistance screening, biological indexing of host plants, epidemiological studies and analysis of plant-pathogen interactions. With the aim of developing a reliable bioassay for CBCVd for hop genotype resistance screening and analysis of CBCVd host range, we compared four different types of inoculum based on RNA and sap extract obtained from CBCVd infected donor plants. Inoculum was mechanically introduced using stem injections or rubbing the inoculum onto leaves treated with carborundum. Results of our study show that the best bioassay for CBCVd is rubbing the sap extract inoculum onto leaves treated with carborundum.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 709772In the frame of scientific conference we present viroid management studies in Slovenia. Until now 4 viroids are known on hop from which Hop latent viroid (HLVd), Hop stunt viroid (HSVd) and Citrus bark cracking viroid (CBCVd) are present in Slovenia. The most harmful is CBCVd which causes severe hop stunt disease. We present epidemiological studies which were focused into study of critical viroids spreading points. These studies significantly contribute in improvement of management strategy and in eradication process. The results were also base for changes in legislation which cover hop viroids in Slovenia and prepositions for EU measures.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 8301689On the invitation of EU standing committee, section Plant health, we presented pest risk analysis (PRA) for hop stunt viroid (HSVd) and citrus bark cracking viroid (CBCVd). PRA was prepared based on new outbreaks of viroids in Slovenia and included several criteria which are important for future status of harmful organism and control measures. Presented PRA was the first case which was until now reported from Slovenia.
F.17 Transfer of existing technologies, know-how, methods and procedures into practice
COBISS.SI-ID: 724108