Laboratory testing for the presence of 8 garlic viruses using serological methods (DAS- and TAS-ELISA) showed high infection rate of plants raised from two propagation material batches of local variety Ptujski jesenski. Meristem culture and in vitro multiplication of the variety Ptujski jesenski were introduced and combined with thermotherapy in the attempt to produce virus-free material. Virus elimination rate showed to be very low. The selection of material to be used for further multiplication or elimination of viruses is therefore critical. Reliable sampling and sensitive detection techniques need to be used for this purpose.
COBISS.SI-ID: 5402472
Results obtained in the frame of two research projects by sampling and testing of local apricot cultivars from various locations for the presence of 'Candidatus Phytoplasma prunorum' and PPV (Plum pox virus) were presented. Samples were collected from 8 trees of variety Pišeška marelica, 2 of Budanjska marelica, 2 of Bela pašta, 3 of Catarji, 10 of Debeli flokaji and 2 of Drobni flokarji. With exception of one tree of variety Pišeška marelica all other trees proved to be infected with 'Candidatus Phytoplasma prunorum', but none of them showed symptoms of phytoplasma infection. Several hypotheses could be postulated to explain the absence of symptoms and were discussed in the presentation.
COBISS.SI-ID: 4729448
Phylogenetically closely related phytoplasmas from the AP group ‘Candidatus Phytoplasma mali’ and ‘Ca. P. prunorum’ are the causal agents of economically important diseases of temperate fruit trees apple proliferation (AP) and European stone fruit yellows (ESFY), respectively. The results of this study demonstrate that in Slovenia the genetic diversity of both phytoplasmas is moderate. Five different ‘Ca. P. mali’ genotypes can be distinguished based on the aceF gene, six genotypes based on the secY gene, three genotypes based on pnp and seven different genotypes based on the imp gene. Based on the multi locus sequence analysis 16 different haplotypes are present in Slovenia. For ‘Ca. P. prunorum’ four genotypes can be discriminated on the basis of the gene aceF, two on the basis of secY, one based on pnp and five based on imp. With a multilocus sequence analysis seven different haplotypes were found
COBISS.SI-ID: 4259407
Results of testing old local apricot cultivars for the presence of phytoplasma using molecular diagnostic methods are presented. ‘Ca. P. prunorum’ was confirmed in 26 out of 27 apricot trees tested. Some of them are older than 50 years and although they are infected, none of the trees show symptoms of the disease. To study the tolerance of these cultivars buds were taken from four infected trees of varities Debeli Flokarji and Catarji and grafted onto Myrobalan 29C rootstock in the spring 2013. All 12 grafted plants developed well in the first year as well in the second year after buding, although four plants showed premature leaf development in the spring 2014. At the end of the second growth year the molecular analyses confirmed the presence of ‘Ca. P. prunorum’ in 10 out of 12 plants.
COBISS.SI-ID: 4672872
Modern hybrid maize varieties dominated for several decades both in Slovenia and elsewhere in the world. Improvement of local varieties is possible by recurrent selection and may in the case of their specific characteristics deserve their cultivation for specific purposes. Presented experiments have been conducted to characterize selected old Slovenian accessions in two regions - in central Slovenia and in Bela Krajina. Yields of corn at both locations and both years were significantly depending on the genotype. 'Rdeča Bohinjka' and partially 'Rumena Bohinjka' and, similarly, the 'LJ 180' had a predominantly low yields but were also the earliest, while modern hybrid variety 'P 9074' and 'Ronaldinio KWS ' had the expected highest yield. Yield of 'P 9074' grown at the Laboratory field of the Biotechnical Faculty was almost twice higher than the second best accession 'Metliška Plut'.
COBISS.SI-ID: 8804729