The formation of a gel coat around xanthan (Xan) tablets, empty or loaded with pentoxifylline (PF), and its release in media differing in pH and ionic strength by NMR, MR imaging, and two release methods were studied. The T1 and T2 NMR relaxation times in gels depend predominantly on Xan concentration; the presence of PF has negligible influence on them. It is interesting that the matrix swelling is primarily regulated by Xan despite high drug loading (25%, 50%). The gastric pH and high ionic strength of the media do not influence the position of the penetration and swelling fronts but do affect the erosion front and gel thickness. The different release profiles obtained in mixing and nonmixing in vitro methods are the consequence of matrix hydration level and erosion at the surface. In water and in diluted acid medium with low ionic strength, the main release mechanism is erosion, whereas in other media (pH 1.2, % % 0.20 M), anomalous transport dominates as was found out by fitting of measured data with theoretical model. Besides the in vitro investigation that mimics gastric conditions, mathematical modeling makes the product development more successful.
COBISS.SI-ID: 4038513
A novel multiparametric magnetic resonance microscopy (MRM) approach was applied to the Slovenian Kraški pršut dry-cured ham samples in order to evaluate its potential for discrimination among biceps femoris and semimembranosus muscle from two hams, differing in processing (salting duration) and thus in water and salt content. The approach is based on apparent diffusion coefficient (ADC) mapping as well as on longitudinal (T1) and transversal (T2) nuclear magnetic resonance relaxation time mapping. Three-dimensional maps were acquired and analyzed by one dimensional (1D) ADC, T1, and T2 distributions as well as by paired two-dimensional ADC-T1, ADC-T2 and T1–T2 distributions. The discriminating potential of the applied MRM approach was confirmed by differences among both 1D and 2D distributions of different ham samples. In addition, distribution peak positions highly correlated with the conventionally determined moisture content.
COBISS.SI-ID: 29066023
In this study we investigated the structural organization of the lens epithelial cells, in particular their basal side which is in contact with the basal lamina. Three techniques, SEM, TEM and confocal microscopy have been employed, each of them showing the same morphological features, the extensions and the entanglements of the epithelial cells cytoplasmic membrane at the border with the capsule. This knowledge was then used to enable handling of the retinal epithelial cells while searching the effect of the laser light through the (micro)spectral detection.
COBISS.SI-ID: 2628780
The submicron characterisation of transient heat-transfer processes and the corresponding processes at interfaces is of great importance in many areas of science and engineering. We developed the detection of temperature on the edge of boiling microbubbles and we transfer our knowledge published in the referenced paper below into the ophthalmology to enable detection of thermal-induced tissue changes via thermal imaging of the temperature field underneath a growing bubble. In this case the bubble did not occur due to nucleate boiling but due to energy release from laser pulse. Fluorescence microspectroscopy have been employed for this detection.
COBISS.SI-ID: 14672155
Tetrahydropyran derivative 1 was discovered in a high-throughput screening campaign to find new inhibitors of mycobacterial InhA. Following initial in-vitro profiling, a structure-activity relationship study was initiated and a focused library of analogs was synthesized and evaluated. This yielded compound 42 with improved antimycobacterial activity and low cytotoxicity. Additionally, the crystal structure of InhA in complex with inhibitor 1 was resolved, to reveal the binding mode and provide hints for further optimization.
COBISS.SI-ID: 4025969