Verticillium wilt (VW) can cause substantial yield loss in hop particularly with the outbreaks of the lethal strain of Verticillium albo-atrum. To elucidate genetic control of VW resistance in hop, an F1 mapping population derived from a cross of cultivar Wye Target, with the predicted genetic basis of resistance, and susceptible male breeding line BL2/1 was developed to assess wilting symptoms and to perform QTL mapping. A significant QTL for VW resistance was detected at LOD 7 on a single chromosomal region on LG03 of both parental maps, accounting for 24.2 to 26.0% of the phenotypic variance. The work provides the basis for exploration of QTL flanking markers for possible use in marker assisted selection. Besides work published in Theoretical and Applied Genetics, two other relevant publications are listed here: 1) MCADAM, Erin L., FREEMAN, Jules S., WHITTOCK, Simon P., BUCK, Emily J., JAKŠE, Jernej, ČERENAK, Andreja, JAVORNIK, Branka, LILIAN, Andrzej, WANG, Cai-Hong, ANDERSEN, Dave, VAILLANCOURT, René E., CARLING, Jason, BEATSON, Ron, GRAHAM, Lawrence, GRAHAM, Donna, DARBY, Peter E., KOUTOULIS, Anthony. Quantitative trait loci in hop (Humulus lupulus L.) reveal complex genetic architecture underlying variation in sex, yield and cone chemistry. BMC genomics, ISSN 1471-2164, 2013, vol. 14, art. no. 360, str. 1-27. http://www.biomedcentral.com/1471-2164/14/360, doi: 10.1186/1471-2164-14-360. [COBISS.SI-ID 7596153] 2) ČERENAK, Andreja, ŠATOVIĆ, Zlatko, JAKŠE, Jernej, LUTHAR, Zlata, CAROVIĆ-STANKO, Klaudija, JAVORNIK, Branka. Identification of QTLs for alpha acid content and yield in hop (Humulus lupulus L.). Euphytica, ISSN 0014-2336. [Print ed.], 2009, vol. 170, no. 1-2, str. 141-154. http://dx.doi.org/10.1007/s10681-009-9920-9, doi: 10.1007/s10681-009-9920-9. [COBISS.SI-ID 5954681]
COBISS.SI-ID: 7466361
V preteklih desetih letih so člani programske skupine razvijali metode indukcije haploidnih in podvojenih haploidnih rastlin pri številnih rastlinskih vrstah, kot so: čebula (Jakše et al., 2010; Duangjit et al., 2013; Foschi et al., 2013; Duangjit et al., 2014), zelje (Rudolf Pilih et al., 2018), oljne buče (Košmrlj et al., 2013), rukola (Leskovšek et el., 2008), kostanj (Ćalić et al., 2013) in Mimulus aurantiacus (Murovec and Bohanec, 2013). Uporabnost (podvojenih) haploidov v kmetijstvu so predstavili v dveh odprtodostopnih virih: poglavju monografske publikacije (Murovec and Bohanec, 2012) in preglednem članku (Murovec, 2013). Članek »Transcriptome sequencing to produce SNP-based genetic maps of onion« opisuje uporabo haploidov v bazičnih genetskih raziskavah. Dve genetsko različni inbridirani liniji čebule (OH1 in 5225) sta bili križani in iz njunih hibridov je bilo pridobljenih 182 ginogenetskih podvojenih haploidov, ki so bili uporabljeni za kartiranje SNP markerjev. V potomstvu je skupno segregiralo 597 SNP-jev, izdelana je bila genetska karta desetih povezanih skupin. Ker so te novi SNP-ji izraženi v regijah genoma, ki se pogosto prepisuje v čebuli pričakujemo, da bodo koristni za gensko kartiranje, za označevanje genov, pri žlahtnjenju s pomočjo genskih markerjev, pri nadzoru kakovosti v semenarstvu in pri genotipizaciji novih sort. JAKŠE, Marijana, HIRSCHEGGER, Pablo, BOHANEC, Borut, HAVEY, Michael J. Evaluation of gynogenic responsiveness and pollen viability of selfed doubled haploid onion lines and chromosome doubling via somatic regeneration. Journal of the American Society for Horticultural Science, ISSN 0003-1062. [Print ed.], 2010, vol. 135, no. 1, str. 67-73. [COBISS.SI-ID 6250105] DUANGJIT, Janejira, WELSH, Kent, WISE, Mitchel L., BOHANEC, Borut, HAVEY, Michael J. Genetic analyses of anthocyanin concentrations and intensity of red bulb color among segregating haploid progenies of onion. Molecular breeding, ISSN 1380-3743. [Tiskana izd.], 2014, vol. 34, iss. 1, str. 75-85. http://dx.doi.org/10.1007/s11032-014-0018-2, doi: 10.1007/s11032-014-0018-2. [COBISS.SI-ID 7848569] FOSCHI, María Laura, MARTÍNEZ, Liliana Estela, PONCE, María Teresa, GALMARINI, Claudio Rómulo, BOHANEC, Borut. Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size = Efecto de la colchicina y del amiprofos-metil en la producción in vitro de plantas dihaploides de cebolla y determinación de la correlación entre el nivel de ploidía y tamano de los estomas. Revista de la Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo, ISSN 0370-4661, 2013, vol. 45, no. 2, str. 155-164, ilustr. [COBISS.SI-ID 7835769] DUANGJIT, J., BOHANEC, Borut, CHAN, Agnes P., TOWN, Christopher, HAVEY, Michael J. Transcriptome sequencing to produce SNP-based genetic maps of onion. Theoretical and Applied Genetics : International journal of plant breeding, ISSN 0040-5752. [Print ed.], 2013, vol. 126, issue 8, str. 2093-2101. http://dx.doi.org/10.1007/s00122-013-2121-x, doi: 10.1007/s00122-013-2121-x. [COBISS.SI-ID 7583609] KOŠMRLJ, Kristina, MUROVEC, Jana, BOHANEC, Borut. Haploid induction in hull-less seed pumpkin through parthenogenesis induced by X-ray-irradiated pollen. Journal of the American Society for Horticultural Science, ISSN 0003-1062. [Print ed.], 2013, vol. 138, no. 4, str. 310-316. [COBISS.SI-ID 7651449] ĆALIĆ, Dušica, BOHANEC, Borut, DEVRNJA, Nina, MILOJEVIĆ, Jelena, TUBIĆ, Ljiljana, KOSTIĆ, Igor, ZDRAVKOVIĆ-KORAĆ, Snežana. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. Trees, ISSN 0931-1890, 2013, vol. 27, no. 3, str. 755-762, ilustr. http://dx.doi.org/10.1007/s00468-012-0830-4, doi: 10.1007/s00468-012-0830-4. [COBISS.SI-ID 7381113] MUROVEC, Jana, BOHANEC, Borut. Haploid induction in Mimulus aurantiacus Curtis obtained by pollination with gamma irradiated pollen. Scientia horticultu
COBISS.SI-ID: 7583609
The research group has also worked in the field of development and technical applications of molecular markers, with emphasize on research and characterization of microsatellite markers for analysis of plant genomes. We have isolated and characterized many microsatellite markers in common sage, which were used for genetic variability study of selected population and for testing of their applicability in cross taxa research. In hops, genic microsatellite markers were developed based on the publicly available sequences and showed their suitability for genetic variability studies and for recombinant mapping efforts in hops. Furthermore, molecular markers in hops were developed, which are based on conserved domains motifs specific for resistance genes and used them for genetic mapping. Interesting field of studies conducted in our group is dioecism in hops and emergence of hermaphrodites. We have shown with application of microsatellite markers that in latter trait, unreduced gametes play an important role. In collaboration with group from Saudi Arabia, we have published common paper on EST markers from date palm. Published papers related to the achievement are listed below: 1. ALFAIFI, Sulieman, KHAN, Muhammad Anas, MIGDADI, Hussein M., JAKŠE, Jernej, AMMAR, Megahed H., EL-HARTY, Ehab H., ALTHAMRAH, Mohammad I., AFZAL, Muhammad, JAVED, Muhammad M., ALGHAMDI, Salem S. Analysis of ESTs from the date palm (Phoenix dactylifera L.) cv. Sukary, an elite variety. Plant omics, ISSN 1836-0661, 2015, vol. 8, issue 5, str. 441-448. http://www.pomics.com/migdadi_8_5_2015_441_448.pdf. [COBISS.SI-ID 8269689] 2. RADOSAVLJEVIĆ, Ivan, SATOVIĆ, Zlatko, JAKŠE, Jernej, JAVORNIK, Branka, GREGURAŠ, Danijela, JUG-DUJAKOVIĆ, Marija, LIBER, Zlatko. Development of new microsatellite markers for Salvia officinalis L. and its potential use in conservation-genetic studies of narrow endemic Salvia brachyodon Vandas. International journal of molecular sciences, ISSN 1661-6596, 2012, vol. 13, issue 9, str. 12082-12093, ilustr. http://dx.doi.org/10.3390/ijms130912082, doi: 10.3390/ijms130912082. [COBISS.SI-ID 7252089] 3. RADOSAVLJEVIĆ, Ivan, JAKŠE, Jernej, JAVORNIK, Branka, SATOVIĆ, Zlatko, LIBER, Zlatko. New microsatellite markers for Salvia officinalis (Lamiaceae) and cross-amplification in closely related species. American journal of botany, ISSN 0002-9122, 2011, vol. 98, no. 11, str. e316-e318, ilustr., doi: 10.3732/ajb.1000462. [COBISS.SI-ID 6874233] 4. MAJER, Aljaž, JAVORNIK, Branka, ČERENAK, Andreja, JAKŠE, Jernej. Development of novel EST-derived resistance gene markers in hop (Humulus lupulus L.). Molecular breeding, ISSN 1380-3743. [Tiskana izd.], 2014, vol. 33, issue 1, str. 61-74. http://dx.doi.org/10.1007/s11032-013-9934-9, doi: 10.1007/s11032-013-9934-9. [COBISS.SI-ID 7654009] 5. KOZJAK, Petra, JAKŠE, Jernej, JAVORNIK, Branka. Isolation and sequence analysis of NBS-LRR disease resistance gene analogues from hop Humulus lupulus L. Plant science, ISSN 0168-9452. [Print ed.], 2009, vol. 176, issue 6, str. 775-782, doi: 10.1016/j.plantsci.2009.02.021. [COBISS.SI-ID 5895289] 6. ŠKOF, Suzana, ČERENAK, Andreja, JAKŠE, Jernej, BOHANEC, Borut, JAVORNIK, Branka. Ploidy and sex expression in monoecious hop (Humulus lupulus). Botany, ISSN 1916-2790. [Tiskana izd.], 2012, vol. 90, no. 7, str. 617-626. http://dx.doi.org/10.1139/b2012-037. [COBISS.SI-ID 7165817] 7. BARUCA ARBEITER, Alenka, HLADNIK, Matjaž, JAKŠE, Jernej, BANDELJ, Dunja. Identification and validation of novel EST-SSR markers in olives. Scientia Agrícola, ISSN 1678-992X, May/June 2017, v. 74, n. 3, str. 215-225, ilustr., doi: 10.1590/1678-992X-2016-0111. [COBISS.SI-ID 8685945]
COBISS.SI-ID: 6353273
The members of a research group are tightly linked to interenational cooperation with different researchers in the field of discovering hop genome. In the study of Howard et al (2011) we are reporting about the first utilization of DArT for hop genotyping, where 730 polymorphic markers from 92 hop accessions were identified. Genetic relationships in hop illustrated by DArT (microarray technology) used in this study coincide with knowledge generated using alternate methods. Levels of genetic diversity were similar in the North American and European groups, but higher in the hybrid group. The markers produced are valuable resource for numerous applications in hop breeding and genetics studies. Furthermore, the same group (McAdam et al, 2013) has linked developed DArT markers with phenotypic data. Pleiotropy and linkage, previously unrecognised in hop, have important implications for molecular selection methods; the selection of particular secondary metabolite profiles using pleiotropic/linked QTL will be challenging due to the difficulty of selecting for specific traits without causing adverse changes to others. The linkage information obtained in this study, based on transferable markers, can be used for comparative mapping, facilitating validation of QTL, crucial to the success of MAS. Additionally, our research group applied selected 4 DArT markers specific for male plants in hop breeding program (Čerenak et al., 2015). Other relevant publications: 1. McAdam Erin L., Freeman Jules S., Whittock Simon P., Buck Emily J., Jakše Jernej, Čerenak Andreja, Javornik Branka, Lilian Andrzej, Wang Cai-Hong, Andersen Dave, Vaillancourt René E., Carling Jason, Beatson Ron, Graham Lawrence, Graham Donna, Darby Peter E., Koutoulis Anthony. BioMed Central; BMC genomics; 2013; Vol. 14, art. no. 360; str. 1-27; Impact Factor: 4.041;Srednja vrednost revije / Medium Category Impact Factor: 2.947; A': 1; WoS: DB, KM 2. ČERENAK, Andreja, JAKŠE, Jernej, KOLENC, Zala, ŠKOF, Suzana, KOUTOULIS, Anthony, WHITTOCK, Simon P., JAVORNIK, Branka. Določanje spola sejancem hmelja z uporabo moško specifičnih molekulskih markerjev = Determination of sex in hop seedlings by using male-specific molecular markers. V: ČEH, Barbara (ur.), et al. Novi izzivi v agronomiji 2015 : zbornik simpozija, Laško, [29. in 30. januar] 2015 = New challenges in agronomy 2015 : proceedings of symposium, [Laško, 2015]. Ljubljana: Slovensko agronomsko društvo. 2015, str. 157-161, ilustr.
COBISS.SI-ID: 6572153
CRISPR-mediated genome editing using the Streptococcus pyogenes Cas9 enzyme is revolutionizing life science by providing new, precise, facile and high-throughput tools for genetic modification by the specific targeting of double-strand breaks in the genome of hosts. The article "New variants of CRISPR RNA-guided genome editing enzymes” describes characteristics of newly developed enzyme systems engineered for genome editing, transcriptional regulation and cellular imaging, such as C2c2 from L. shahii; Cas9 from F. novicida, S. aureus, S. thermophiles and N. meningitidis; Cpf1 from F. novicida, Acidaminococcus and Lachnospiraceae; nickase, split, enhanced and other Cas9 variants from S. pyogenes; catalytically inactive SpCas9 linked to various nuclease or gene-regulating domains and possibilities they offer for plants. The findings will enable us to choose the optimal genome editing system and delivery method for plant species involved in our programme, since our previous experiments showed that some of them (Humulus lupulus and Cannabis sativa) are less responsive in tissue culture and as such not suitable for stable transformation with A. tumefaciens. Moreover, the new genome editing variants will enable us to develop new genome editing approaches that will not involve the use of transgenesis.
COBISS.SI-ID: 8700281
The European Union (EU) has for many years suffered from a dysfunctional voting procedure when it comes to the authorization of genetically modified (GM) crops to be commercially cultivated in EU countries. Several countries regularly demonstrate a voting behavior that seems politically rather than scientifically motivated. To overcome the problems of this procedure, several experts are urging the European Commission to develop legislation that will allow EU countries to individually authorize the cultivation of GM crop varieties that have passed EU risk assessment. This would allow countries to adopt specific crop traits according to their needs. Two years ago, a new legislation gave individual EU countries the right to prohibit the cultivation of GM crops despite EU-level authorization, Slovenia was among first that adapted such legislation. This effectively moved away from the harmonization objective of the GMO Directive. For consistency, countries should also have the corresponding right to authorize the cultivation of GM crops. In the article published in Nature Biotechnology authors proposed, that the risk assessment procedure should remain collective as it is today, under the auspices of the European Food Safety Authority, while member states can individually declare to use or not already registered GMO varieties. Authors believe that such approach would show enable countries that so desire to allow the application of crop traits that will for example reduce pesticide use, provide gluten-free cereals, improve the nutritional and health-promoting qualities of our food, and much more.
COBISS.SI-ID: 8902265
Measurement of gene expression with RTq-PCR are highly sensitive in detecting differences in the abundance of mRNA, any source of variation, including sampling errors, template quality and amplification efficiency, biases the result. The normalization of the results of RT-qPCR by using internal reference genes is therefore essential for the accuracy of RT-qPCR analysis. The characteristic of the proper internal reference is that its gene expression level needs to remain constant under varying experimental conditions. Our recent work included the reference gene evaluation of two species – hops and olives, for which the experimental conditions were different. The combinations of best reference genes identified in the studies were further used for normalization of RT-qPCR data of the genes of interest implicated in either biotic stress of hop or in the developmental stages of olives. Similar approaches were applied on identification of hop transcriptomes during infection with different viroids their differential expression and utilization in biotic stress studies. Other articels related to this topic: 1. REŠETIČ, Tjaša, ŠTAJNER, Nataša, BANDELJ, Dunja, JAVORNIK, Branka, JAKŠE, Jernej. Validation of candidate reference genes in RT-qPCR studies of developing olive fruit and expression analysis of four genes involved in fatty acids metabolism. Molecular breeding, ISSN 1380-3743. [Tiskana izd.], 2013, vol. 32, issue 1, str. 211- 222. http://dx.doi.org/10.1007/s11032-013-9863-7, doi: 10.1007/s11032-013-9863-7. [COBISS.SI-ID 7527801] 2. CREGEEN, Sara, RADIŠEK, Sebastjan, MANDELC, Stanislav, TURK, Boris, ŠTAJNER, Nataša, JAKŠE, Jernej, JAVORNIK, Branka. Different gene expressions of resistant and susceptible hop cultivars in response to Infection with a highly aggressive strain of Verticillium albo-atrum. Plant molecular biology reporter, ISSN 0735-9640, 2015, vol. 33, iss. 3, str. 689-704, doi: 10.1007/s11105-014-0767-4. [COBISS.SI-ID 7992953] 3. POKORN, Tine, RADIŠEK, Sebastjan, JAVORNIK, Branka, ŠTAJNER, Nataša, JAKŠE, Jernej. Development of hop transcriptome to support research into host-viroid interactions. PloS one, ISSN 1932-6203, Sep. 2017, vol. 12, iss. 9, 0184528. https://doi.org/10.1371/journal.pone.0184528. [COBISS.SI-ID 8793977]
COBISS.SI-ID: 7653753
Newly emerging or re-emerging diseases are a constant and significant threat to agricultural production, so prompt and accurate identification of the causative agents is required for rapid and appropriate disease management. Classical methods of pathogen detection can be successfully supplemented by next-generation sequencing (NGS), whereby sequence analysis can help in the discovery of new or emerging diseases. In 2007, hop growers in Slovenia reported the appearance of severely stunted hop plants, a phenomenon that spread rapidly within hop gardens and among farms. Classical diagnostic methods were unable to detect a new pathogen; therefore, single step high-throughput parallel sequencing of total RNA and small RNAs from plants with and without symptoms was employed to identify a novel pathogen. The sequences were assembled de novo and also mapped to reference genomes, resulting in identification of a novel sequence of Citrus bark cracking viroid (CBCVd) in the stunted hop plants. Furthermore, the presence of this novel pathogen on hop was confirmed by RT-PCR analysis of 59 plants with symptoms from 15 hop gardens, representing the main outbreak locations identified by systematic disease monitoring, and small RNA Illumina sequencing of the bulked RNA sample. The high infectivity of the newly identified CBCVd was also confirmed by biolistic inoculation of two hop cultivars, which developed aggressive symptoms in controlled conditions. This study shows the feasibility of deep sequencing for the identification of causative agents of new diseases in hop and other plants. Described paper presents completely new approach of molecular diagnostics. In the past we have been extremely successful in diagnostics and analysis of various plant pathogens and improving of diagnostics procedures: 1) GUČEK, Tanja, TRDAN, Stanislav, JAKŠE, Jernej, JAVORNIK, Branka, MATOUŠEK, Jaroslav, RADIŠEK, Sebastjan. Diagnostic techniques for viroids. Plant Pathology, ISSN 0032-0862, 2017, vol. 66, iss. 3, str. 339-358, doi: 10.1111/ppa.12624. [COBISS.SI-ID 8472953] 2) MATOUŠEK, Jaroslav, SIGLOVÁ, Krystina, JAKŠE, Jernej, RADIŠEK, Sebastjan, BRASS, Joseph R. J., TSUSHIMA, T., GUČEK, Tanja, DURAISAMY, Ganesh Selvaraj, SANO, Teruo, STEGER, Gerhard. Propagation and some physiological effects of Citrus bark cracking viroid and Apple fruit crinkle viroid in multiple infected hop (Humulus lupulus L.). Journal of Plant Physiology, ISSN 0176-1617, 2017, vol. 213, str. 166-177. http://dx.doi.org/10.1016/j.jplph.2017.02.014, doi: 10.1016/j.jplph.2017.02.014. [COBISS.SI-ID 8709241] 3) POKORN, Tine, RADIŠEK, Sebastjan, JAVORNIK, Branka, ŠTAJNER, Nataša, JAKŠE, Jernej. Development of hop transcriptome to support research into host-viroid interactions. PloS one, ISSN 1932-6203, Sep. 2017, vol. 12, iss. 9, 0184528. https://doi.org/10.1371/journal.pone.0184528. [COBISS.SI-ID 8793977] 4) GRIL, Tjaša, CELAR, Franci Aco, JAVORNIK, Branka, JAKŠE, Jernej. Fluorescent AFLP fingerprinting of Monilinia fructicola = Fluoreszenz-AFLP-Fingerprinting von Monilinia fructicola. Journal of plant diseases and protection : scientific journal of the German phytomedical society (DPG), ISSN 1861-3829. [Print ed.], 2010, vol. 117, no. 4, str. 168-172. [COBISS.SI-ID 6346105] 5) MUNDA, Alenka, RADIŠEK, Sebastjan, ŠUŠTAR VOZLIČ, Jelka, JAVORNIK, Branka. Genetic variability of Colletotrichum lindemuthianum isolates from Slovenia and resistance of local Phaseolus vulgaris germplasm. Journal of plant diseases and protection : scientific journal of the German phytomedical society (DPG), ISSN 1861-3829. [Print ed.], 2009, letn. 116, št. 1, str. 23-29. [COBISS.SI-ID 2908264] 6) GRIL, Tjaša, CELAR, Franci Aco, MUNDA, Alenka, JAVORNIK, Branka, JAKŠE, Jernej. AFLP analysis of intraspecific variation between Monilinia laxa isolates from different hosts. Plant disease, ISSN 0191-2917, dec. 2008, vol. 92, no. 12, str. 1616-1624. http://dx.doi.org/10.1094/PDIS-92-12-1616. [COBISS.SI-ID 5743481] 7) LATINOVIĆ
COBISS.SI-ID: 8056185
The interactions between the phytopathogenic fungus Verticillium nonalfalfae and hops were studied at transcriptomic and proteomic levels in two varieties of hops with different sensitivity to disease, separately in roots and aboveground plant parts and at different time intervals. The purpose of the studies was to discover cellular processes and molecular mechanisms leading to the disease resistance and to identify fungal proteins crucial for the development of the disease. We have revealed that sensitive plants at the transcriptional level respond vigorously, as various immune and defense reactions are activated, however, these do not contribute to the protection of hops against the disease. Resistant plants respond to infection with reduced expression of photosynthesis-related genes, but increased expression of genes involved in biosynthesis of terpenoids, cutin, cell wall biogenesis, and the development of lateral roots. In the xylem sap of infected plants, we have discovered two highly represented fungal proteins, which significantly affect development of disease in hop. We have also published two protocols, which will aid in further identification and characterization of the fungal proteins involved in the pathogenesis in hops and model plants. A major contribution to the functional characterization of fungal proteins is the recent publication of the reference fungal genome and the genomes of five fungal strains with different geographical origin and a varying degree of aggressiveness. Other publications related to the described achivement: 1) FLAJŠMAN, Marko, MANDELC, Stanislav, RADIŠEK, Sebastjan, ŠTAJNER, Nataša, JAKŠE, Jernej, KOŠMELJ, Katarina, JAVORNIK, Branka. Identification of novel virulence - associated proteins secreted to xylem by Verticillium nonalfalfae during colonization of hop plants. Molecular plant-microbe interactions, ISSN 0894-0282, 2016, vol. 29, no. 5, str. 362-373, doi: 10.1094/MPMI-01-16-0016-R. [COBISS.SI-ID 8365945] 2) PROGAR, Vasja, JAKŠE, Jernej, ŠTAJNER, Nataša, RADIŠEK, Sebastjan, JAVORNIK, Branka, BERNE, Sabina. Comparative transcriptional analysis of hop responses to infection with Verticillium nonalfalfae. Plant cell reports, ISSN 0721-7714. [Print ed.], 2017, vol. 36, iss 10, str. 1599-1613, doi: 10.1007/s00299-017-2177-1. [COBISS.SI-ID 8755577] 3) JELEN, Vid, DE JONGE, Ronnie, VAN DE PEER, Yves, JAVORNIK, Branka, JAKŠE, Jernej. Complete mitochondrial genome of the Verticillium-wilt causing plant pathogen Verticillium nonalfalfae. PloS one, ISSN 1932-6203, Feb. 2016, vol. 11, iss. 2, e0148525. https://doi.org/10.1371/journal.pone.0148525, doi: 10.1371/journal.pone.0148525. [COBISS.SI-ID 8358265] 4) JAKŠE, Jernej, JELEN, Vid, RADIŠEK, Sebastjan, DE JONGE, Ronnie, MANDELC, Stanislav, MAJER, Aljaž, CURK, Tomaž, ZUPAN, Blaž, THOMMA, Bart P. H. J., JAVORNIK, Branka. Genome sequence of a lethal strain of xylem-invading Verticillium nonalfalfae. Genome announcements, ISSN 2169-8287, 2018, vol. 6, iss. 2, str. 1-2, e01458-17. http://genomea.asm.org/content/6/2/e01458-17.full, doi: 10.1128/genomeA.01458-17. [COBISS.SI-ID 8903033] 5) FLAJŠMAN, Marko, RADIŠEK, Sebastjan, JAVORNIK, Branka. Pathogenicity assay of Verticillium nonalfalfae on hop plants. Bio-protocol, ISSN 2331-8325, 2017, vol. 7, iss. 6, str. 1-10 (2171), ilustr. http://bio-protocol.org/pdf/Bio-protocol2171.pdf, doi: 10.21769/BioProtoc.2171. [COBISS.SI-ID 8734073] 6) FLAJŠMAN, Marko, MANDELC, Stanislav, RADIŠEK, Sebastjan, JAVORNIK, Branka. Xylem sap extraction method from hop plants. Bio-protocol, ISSN 2331-8325, 2017, vol. 7, iss. 6, str. 1-11 (2172), ilustr. http://bio-protocol.org/pdf/Bio-protocol2172.pdf, doi: 10.21769/BioProtoc.2172. [COBISS.SI-ID 8733817]
COBISS.SI-ID: 8365945
Genetic variability and diversity has been widely studied with the tools of various molecular markers for broad range of different species and cultivars or genotypes within species. The obtained results have assisted in various studies like assessment of the origins of different crops and their molecular phylogeny; genetic structures and the origin of germplasm; evaluation of accessions in gene banks and assessment of relationships among varieties. Great importance of marker analyses was the identification of molecular markers that correlates with observed trait or phenotypic variation and is useful for mapping of QTLs and identification of markers linked to the sex of the plants with great applicative value in the breeding processes. List of 14 most relevant publications related to this topic: 1. RADOSAVLJEVIĆ, Ivan, JAKŠE, Jernej, SATOVIĆ, Zlatko, JAVORNIK, Branka, JANKOVIČ, Ivana, LIBER, Zlatko. New microsatellite markers for Campanula pyramidalis (Campanulaceae) and cross-amplification in closely related species. Applications in plant sciences, ISSN 2168-0450, Mar. 2015, vol. 3, iss. 3, str. 1-3 (1400117), doi: 10.3732/apps.1400117. [COBISS.SI-ID 8120953] 2. BARUCA ARBEITER, Alenka, JAKŠE, Jernej, BANDELJ, Dunja. Paternity analysis of the olive variety "Istrska belica" and identification of pollen donors by microsatellite markers. The scientific world journal, ISSN 1537-744X, 2014, vol. 2014, art. ID 208590, str. 1-6. http://dx.doi.org/10.1155/2014/208590, doi: 10.1155/2014/208590. [COBISS.SI-ID 1536576708] 3. ORAŽEM, Petra, ŠTAJNER, Nataša, BOHANEC, Borut. Effect of X-ray irradiation on olive shoot culture evaluated by morphological measurements, nuclear DNA content and SSR and AFLP markers. Trees, ISSN 0931-1890, 2013, vol. 27, issue 6, str. 1587-1595, ilustr.http://link.springer.com/content/pdf/10.1007%2Fs00468-013-0906-9.pdf, doi: 10.1007/s00468-013-0906-9. [COBISS.SI-ID 7652217] 4. RADOSAVLJEVIĆ, Ivan, JAKŠE, Jernej, JAVORNIK, Branka, SATOVIĆ, Zlatko, LIBER, Zlatko. New microsatellite markers for Salvia officinalis (Lamiaceae) and cross-amplification in closely related species. American journal of botany : official publication of the Botanical Society of America, ISSN 0002-9122, 2011, vol. 98, no. 11, str. e316-e318, ilustr., doi: 10.3732/ajb.1000462. [COBISS.SI-ID 6874233] 5. HIRSCHEGGER, Pablo, JAKŠE, Jernej, TRONTELJ, Peter, BOHANEC, Borut. Origins of Allium ampeloprasum horticultural groups and a molecular phylogeny of the section Allium (Allium: Alliacea). Molecular phylogenetics and evolution, ISSN 1055-7903, 2010, vol. 54, no. 2, str. 488-97. http://dx.doi.org/10.1016/j.ympev.2009.08.030, doi: 10.1016/j.ympev.2009.08.030. [COBISS.SI-ID 6122873] 6. CAROVIĆ-STANKO, Klaudija, LIBER, Zlatko, BESENDORFER, Višnja, JAVORNIK, Branka, BOHANEC, Borut, KOLAK, Ivan, SATOVIĆ, Zlatko. Genetic relations among basil taxa (Ocimum L.) based on molecular markers, nuclear DNA content, and chromosome number. Plant systematics and evolution : continuation of Österreichische Botanische Zeitschrift, ISSN 0378-2697, 2010, vol. 285, no. 1-2, str. 13-22, ilustr., doi: 10.1007/s00606-009-0251-z. [COBISS.SI-ID 6214521] 7. ŠTAJNER, Nataša, JAKŠE, Jernej, JAVORNIK, Branka, MASUELLI, R. W., MARTÍNEZ, L. E. Highly variable AFLP and S-SAP markers for the identification of 'Malbec' and 'Syrah' clones. Vitis, ISSN 0042-7500, 2009, letn. 48, no. 3, str. 145-150. [COBISS.SI-ID 6051961] 8. JAKŠE, Jernej, ŠTAJNER, Nataša, KOZJAK, Petra, ČERENAK, Andreja, JAVORNIK, Branka. Trinucleotide microsatellite repeat is tightly linked to male sex in hop (Humulus lupulus L.). Molecular breeding, ISSN 1380-3743. [Tiskana izd.], 2008, vol. 21, no. 2, str. 139-148. [COBISS.SI-ID 5111417] 9. ŠTAJNER, Nataša, RUSJAN, Denis, KOROŠEC-KORUZA, Zora, JAVORNIK, Branka. Genetic characterization of old Slovenian grapevine varieties of Vitis vinifera L. by microsatellite genotyping. American journal of enology and viticulture, ISSN 0002-9254. [Print ed.], 2011, vo
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