Based on the 10-year-long research of phytoplasmal diseases of grapevine by the project leader Marina Dermastia, as well as numerous publication of M.D. and co-workers on this topic, in 2017 the Springer publishing house published a scientific monograph with the review of this research. The editor invited to co-edit the book two project members, Assunta Bertaccini and Nataša Mehle. The monograph have been downloaded 1300-times and have several citations. The monograph is classified as an outstanding achievement by the Slovenian Research Agency.
COBISS.SI-ID: 4184399
A conceptual model of general plant pathology – a disease triangle for describing interactions among the host plant, the pathogen and the environment was applied to generate a proof-of-concept statistical model for disease triangle using original experimental data and different statistical and data mining approaches for a selected system of ‘Ca. P. solani’ infection of cv. ‘Chardonnay’ grapevine plants. We showed that this first de facto representation of the disease triangle is useful for showing disease dynamics over several years and could be applied to other plant-pathogen systems. The paper has been 1200-times downloaded, it has been cited and mentioned at all recent related conferences.
COBISS.SI-ID: 4396623
Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenols and polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and interfere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabolism, while based on our recently published one for quantitative measurement of activities using coupled spectrophotometric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grapevine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteria 'Candidatus Phytoplasma solani’, known to alter carbohydrate metabolism in grapevine. The described adaptations may be useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inherently high levels of phenolic compounds.
COBISS.SI-ID: 4151631
In an integrated study of the FDp grapevine infection under natural conditions in the vineyard, the seasonal transcriptional profiles of 14 genes selected from various metabolic pathways are followed. The study shows a new association of the SWEET17a vacuolar transporter of fructose with pathogens. The first non-targeted metabolome analysis of the infected grapevine identified 22 significantly changed compounds. Several metabolites corroborated the gene expression study. Detailed investigation of the dynamics of carbohydrate metabolism. The data support the conclusion that FDp infection inhibits phloem transport. The present study also revealed expression of VvDMR6 (identified by our statistical modeling) early in the growing season, before the development of symptoms of FDp infection, to be potentially applied to early diagnosis of grapevine yellows.
COBISS.SI-ID: 3870031