A protocol for activity assays for enzymes of primary carbohydrate metabolism is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grapevine leaf. As a case study the protocol to grapevine leaf samples infected with plant pathogenic bacteria 'Candidatus Phytoplasma solani, known to alter carbohydrate metabolism in grapevine, was applied.
COBISS.SI-ID: 4151631
In an integrated study the FDp-grapevine interaction in infected grapevines of cv. "Modra frankinja" under natural conditions in the vineyard was explored. In FDp-infected leaf vein-enriched tissues, the seasonal transcriptional profiles of 14 genes selected from various metabolic pathways showed an FDp-specific plant response compared to other grapevine yellows and uncovered a new association of the SWEET17a vacuolar transporter of fructose with pathogens. Non-targeted metabolome analysis from leaf vein-enriched tissues identified 22 significantly changed compounds with increased levels during infection. Detailed investigation of the dynamics of carbohydrate metabolism revealed significant accumulation of sucrose and starch in the mesophyll of FDp-infected leaves, as well as significant up-regulation of genes involved in their biosynthesis. In addition, infected leaves had high activities of ADP-glucose pyrophosphorylase and, more significantly, sucrose synthase.
COBISS.SI-ID: 3870031
Non-targeted metabolome analysis from leaf vein-enriched tissues of grapevine plants cv. ‘Chardonnay’ infected with ‘Ca. P. solani’ identified 36 annotated or putatively annotated compounds that differed significantly between infected and uninfected samples. The study proved on the metabolome level that infection with phytoplasma significantly affects the carbohydrate metabolism.
COBISS.SI-ID: 3807311