This publication presents a complex and comprehensive inventory of the genetic resources of the beans at the Slovenian Plant Genetic Bank of the Agricultural Institute of Slovenia. Using morphometric parameters, we evaluated the collection of genetic resources of ordinary (Phaseolus vulgaris L.) and runner beans (Phaseolus coccineus L.). With the prescribed descriptors (UPOV, CPVO, IBPGR, PHASELIEU) for seeds of the species Phaseolus sp. and extensive picture material we characterize and describe 955 common beans and 47 accessions of runner beans. The book is intended for a wider circle of readers, from gardeners, farmers and students to breeders and researchers, who can thus acquaint themselves with the collected heredity and a large collection stored for future generations at the Agricultural Institute of Slovenia. We dedicate this book to all researchers and researchers who have worked and are still active in the field of genetic resources of agricultural plants and thus take care of the preservation of the natural and cultural heritage in Slovenia.
F.27 Contribution to preserving/protecting natural and cultural heritage
COBISS.SI-ID: 297596928The aim of the study was to determine the stability of productivity properties of the local accessions of Phaseolus vulgaris L, in the two periods of technological maturity (green pods and grain) depending on climatic conditions and the region where it is grown. Investigations were carried out on 13 accessions of subspecies Phaseolus vulgaris L. ssp. volubilis at two different locations in the northwest part of the RS/BiH (Aleksandrovac and Kostajnica) during two years. Characterization of properties of productivity (number of pods per plant, length and width of the pod, plant height, length, width, thickness and weight of seeds, number of seeds per pod) is done by IBPGR descriptors for species Phaseolus vulgaris.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 5380968Thousands of landraces, old and modern cultivars are maintained in gene banks across the European continent. According to basic multi-crop passport descriptors and seed characteristics, we selected over 800 accessions with distinct genotypes, covering diverse environments from different parts of the European continent following the line East to West and East to South. To evaluate their genetic diversity, we applied 33 species specific SSR markers covering all linkage groups among Phaseolus vulgaris genome. Four strategies were used to construct core collection combining different parameters of genetic variability. Out of the entire collection, 63 highly diverse accessions were selected for core collection on the basis of their genetic structure, without the geographic origin preference. Final core collection represents valuable source of important traits and (multiple) alleles with potential to carry biotic and abiotic stress resistance, having a great value for common bean breeding programmes.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 5454440Common bean (Phaseolus vulgaris L.) was introduced in Europe from both Mesoamerican and Andean centres of origin and has been cultivated in central and south east Europe for centuries. The first objective of the study was to evaluate genetic diversity and the population structure of 286 accessions from five former Yugoslav republics, using 13 simple sequence repeat markers. The second objective was to improve our understanding of the pathways of dissemination in central and south east Europe. On the basis of the scored number of migrants, we concluded that the most intensive gene flow in the region exists in Bosnia and Herzegovina. This could indicate that common bean was introduced into the western Balkans mainly from the Mediterranean Basin.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 5428584Common bean (Phaseolus vulgaris L.) is the most important edible food legume for direct human consumption in the world as it represents a valuable source of components with nutritional and health benefits. Thus, it is a crop featured in breeding programs worldwide, including Agricultural Institute of Slovenia. Genetic diversity of different common bean genetic resources has been evaluated in numerous studies using different DNA markers. Information on genetic diversity of common bean from Central and Eastern Europe is scarce; therefore our study was a basis for construction common bean core collection (CC) and final collection (FC) using Central and East European germplasm. Regarding to basic multi-crop passport descriptors and seed characteristics including geographic origin, biological status, ancestral data, phenotypic seed characteristics and different phaseolin type (corresponding Andean/Mesoamerican origin), 782 accessions (ACCs) from 9 gene banks and 12 geographic origins were selected and observed as a whole collection (WC). Genotyping procedure using 33 genome-specific nSSR and EST-SSR markers was performed to assess genetic background of WC. Regarding to diversity parameters and genetic structure, we proposed CC encompassing 63 accessions, representing global genetic diversity of WC - all of 782 ACCs from different geographic origins. Additionally, we identified 14 standard genotypes/known varieties expressing desirable traits of interest to construct final CC. Altogether, 77 genotypes from CC were further evaluated on different levels combining agronomic, genetic and nutritional traits of importance including: I) morphological/phenotypical evaluations to test actual agronomic traits/response including abiotic/biotic resistance potential under field conditions; II) morphometric analysis of seeds regarding to Community Plant Variety Office-Technical Protocol/Phaselieu common bean seed descriptors; III) marker-assisted evaluation trait-specific selection for favourable traits (bean common mosaic virus, bean rust, anthracnose and angular leaf spot resistance; drought/heat tolerance; low phosphorus uptake and root morphology; high zinc and iron content; earliness and high yield) applying 75 trait-related DNA markers including KASP_SNP markers (24), nSSRs (35), SCAR (5), EST-SSR (2), CAPS (1) and locus-specific PCR based markers (8); IV) biochemical analysis including multi-elemental composition, fats, proteins and phytic acids. After marker-assisted evaluation and complex combination of the results obtained from all four evaluation levels using rating system, we established FC of 12 elite common bean ACCs representing superior potential for breeding applications.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 5581928