In the study, genetic diversity of 119 accessions of Common Bean (Phaseolus vulgaris L.) from five former Yugoslav republics constituting Western Balkans was assessed by 13 microsatellite markers, that efficiently distinguish between bean genotypes belonging to the Andean or the Mesoamerican gene pool. We found that Andean genotypes prevail in all studied countries by the frequency larger than 60%, except Macedonia, where the two gene pools are represented evenly. This could indicate that Common Bean was introduced into Western Balkans mainly from Mediterranean Basin. A moderate but notable shift in the pattern of genetic diversity on the southern boundaries might be explained by the influence of surrounding regions, e.g. Bulgaria and Greece, which were reported elsewhere to comprise distinctive germplasm.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 4977256The results of the research work of molecular and morpho-physiological adaptation of common bean to drought were presented at the congress. A differential gene expression study, conducted on leaves of eight genotypes at different levels of dehydration, revealed that drought induced alteration of 15 drought-responsive transcripts. Using proteomic analysis of leaves of two cultivars differing in their response to drought drought-responsive proteins were identified and grouped in functional categories, including metabolic proteins, cell defence/stress proteins, proteins involved in photosynthesis and proteins with unknown functions. The population of 80 recombinant inbred lines (RILs) of the F8 generation, derived by a cross between a more and a less sensitive cultivar was used to develop a genetic map. Polymorphic markers were arranged into 11 linkage groups (LGs) from the common bean consensus map and two additional, unclassified LGs. QTL analysis revealed a linkage between markers and QTLs for days to flowering and single seed mass in LG1 and LG9 from the consensus map. These markers will be used along with others identified in the research of the core collection established within the current project.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 5112936The presentation about short tandem repeats and its direct or indirect impact to agriculture, was performed on the plant genomic seminar. According to different important plant species (including common bean), we presented the application of specific marker system to investigate the presence of genes/QTLs inside the genomes, which are correlated with specific agronomically important traits (biotic/abiotic stress, characteristics for some nutritional components, some phenotypically expressed characteristics).
B.04 Guest lecture
COBISS.SI-ID: 5028712