The major contributions of the research program to the science are the newly developed and highly sensitive analytical methods, which are based on TLS spectrometry. They enable observation of new phenomena and processes in our environment, which could not be investigated in the past because of the lack of sufficiently sensitive methods. Such examples include on-line observation of cell lysis during algal blooms and investigations of parallel xantophyll cycles in phytoplankton. In addition, some fundamental results such as the established effects of organophosphates on investigated organisms (Porcelio scaber, Isopods), the mechanisms and kinetics of degradation of pollutants in natural environments or under the influence of various sources of radiation, as well as the effects of environmental factors and chemicals on the content of anthocyanins in fruits, are important from the scientific point of view. The following achievements are noteworthy: - Development of an IC-TLS method for simultaneous determination of Cr(III) and Cr(VI) species and its application for analysis of drinking water - Development of an optothermal AChE biosensor - Determination of sub-lethal toxicity of OP pesticides for terrestrial isopods - Determination of key indicators of OP toxicity in terrestrial isopods and their application for biomonitoring of environmental pollution with OP and carbamate pesticides - Determination of the mechanism and efficiency of degradation of diazinon with laser light, X-rays and sunlight - Development of HPLC-TLS technique for determination of beta carotene and other carrotenoids in vegetable oils and quality assessment - Development of gradient elution HPLC-TLS for detection of pigments in plants - Development of HPLC method for determination of anthocyanins and hydroxi-cynnamic acids - Determination of the effects of environmental factors and chemicals on the content of anthocyanins in sweet cherries - Development of a double dual-beam TLS spectrometer for studies of cell lysis in phytoplankton - TLS detection of minor pigments in parallel xantophyll cycles and the effects of environmental parameters in some phytoplankton species - Development of a novel model for determination of aerosol transport in the atmosphere, based on two dimensional LIDAR measurements