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Projekti / Programi vir: ARIS


Raziskovalna dejavnost

Koda Veda Področje Podpodročje
4.06.00  Biotehnika  Biotehnologija   

Koda Veda Področje
3.04  Medicinske in zdravstvene vede  Medicinska biotehnologija 
Ključne besede
proteinske nanopore, detekcija, visoko zmogljivo zaznavanje, histoni
Vrednotenje (pravilnik)
Raziskovalci (8)
št. Evidenčna št. Ime in priimek Razisk. področje Vloga Obdobje Štev. publikacijŠtev. publikacij
1.  15686  dr. Gregor Anderluh  Biokemija in molekularna biologija  Vodja  2020 - 2023  960 
2.  31814  Urška Dečko    Tehnični sodelavec  2020 - 2021 
3.  35382  dr. Matic Kisovec  Biokemija in molekularna biologija  Raziskovalec  2020 - 2023  78 
4.  13627  dr. Franci Merzel  Računalniško intenzivne metode in aplikacije  Raziskovalec  2020 - 2023  203 
5.  12048  dr. Marjetka Podobnik  Biokemija in molekularna biologija  Raziskovalec  2020 - 2023  313 
6.  53732  Marija Srnko  Biokemija in molekularna biologija  Raziskovalec  2020 - 2023  22 
7.  38479  dr. Aleksandra Šakanović  Nevrobiologija  Raziskovalec  2021 - 2022  24 
8.  50369  Gašper Šolinc  Biokemija in molekularna biologija  Tehnični sodelavec  2020 - 2023  21 
Organizacije (1)
št. Evidenčna št. Razisk. organizacija Kraj Matična številka Štev. publikacijŠtev. publikacij
1.  0104  Kemijski inštitut  Ljubljana  5051592000  21.377 
Biological nanopores are increasingly popular in molecular biotechnology, synthetic biology and medical applications, due to their usefulness in sensing. Nanopores are extremely stable structures and are formed by pore-forming proteins in lipid membranes. The final pore has defined molecular properties, such as the diameter of the pore, which is in range of several nanometers. Due to their high stability they can be further manipulated and used for sensing applications, where ionic currents that pass through the single pore can be measured. The method can detect interaction of various analytes with the pore lumen. The binding event or occlusion of the pore by the analyte change ionic currents and these blockades are characteristic for a particular analyte. The method is extremely sensitive and the use of nanopores in detection of various analytes, such as small molecules, peptides, proteins, RNA and DNA, has gained a momentum in recent years, with the nicest example of DNA sequencing. So far only a handful of pores exist and novel pores with changed properties are crucially needed for sensing. In this project we will focus on development of sensing approaches of novel nanopore based on the pore forming protein from a coral. The following research objectives will lead to a new engineered nanopore and sensing application for medically important proteins: i) we plan to develop nanopore for stable incorporation into artificial membrane system that will allow high-throughput sensing; ii) we plan to develop nanopore for sensing of medical relevant proteins and iii) we will develop sensing applications which will be able to resolve highly similar peptides and proteins that are post-translationally modified. The development of nanopores will significantly advance the field of macromolecular sensing. We will provide novel pore that will be available for sensing of various other analytes in a high-throughput manner, which is currently lacking and needed for efficient identification of medically important proteins in a fast and facile manner.
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