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Projects
Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection
Research activity
| Code | Science | Field |
|---|---|---|
| P004 | Natural sciences and mathematics | Biochemistry, Metabolism |
| P305 | Natural sciences and mathematics | Environmental chemistry |
| P310 | Natural sciences and mathematics | Proteins, enzymology |
| P320 | Natural sciences and mathematics | Nucleic acids, protein synthesis |
| T490 | Technological sciences | Biotechnology |
Keywords
Enzymes, immobilization, food, bioethanol, antioxidants, fermentation
Organisations (3) , Researchers (1)
0011 University of Belgrade, Faculty of Chemistry
| no. | Code | Name and surname | Research area | Role | Period | No. of publicationsNo. of publications |
|---|---|---|---|---|---|---|
| 1. | 01961 | Biochemistry, Metabolism | Head | 2011 - 2019 | 48 |
0095 University of Belgrade, Institute of Chemistry, Technology and Metallurgy - National Institute of the Republic of Serbia
0257 Innovation Center, Faculty of Chemistry in Belgrade Ltd (IC)
Abstract
Research focus will be to study and optimize the conditions for production and purification of enzymes from plant, animals and microorganisms and their application as pure or in complex enzyme mixtures, soluble or immobilized, in food biotechnology, pure and cheap bioethanol production and environmental protection. Selected microorganism strains will be grown in submerged and solid state fermentations (SF and SSF, respectively). Wild type and recombinant enzymes will be purified to homogeneity by chromatographic and electrophoretic techniques and characterized in details by biochemical and biophysical methods. Previously started research concerning the improvement of enzyme stability by chemical modifications and immobilizations (including newly synthesised supports) for food biotechnology and environmental protection will be continued. Improvement of food quality using enzymes and small molecules and plant fibres will be studied by analysing food nutritive and energetic value, variation in contents of soluble and insoluble proteins, variations in amino acid content and antioxidative capacity. Condition for the extraction of proteins and other polymers and its hydrolysis to fermentable or eatable molecules will be explored. Oxydases will be used for detoxification of contaminated water through the polymerisation-precipitation reactions. Mechanism of detoxification will be examined by biological assays and interactions with DNA and proteins.