MOLECULAR AND PHYSIOLOGICAL BIOMONITORING OF AEROBIC ORGANISMS BASED ON THE DETERMINATION OF BIOCHEMICAL BIOMARKERS OF OXIDATIVE STRESS

Molecular and physiological biomonitoring based on the antioxidant defense enzyme activities in aerobic organisms has shown an advantage over classical methods of ecotoxicological biomonitoring. Quantitative and qualitative analysis of enzyme components of the system in different aerobic organisms: aquatic - fishes, mussels, snails, rodents - rats, pathophysiological states - preeclampsia, celiac disease and Heliobacter pylory infection, may be used in ecotoxicological biomonitoring of environmental pollution, mathematical modeling of the system, including tissues specificities and seasonal variations. Expected results of the project would be of great significance in providing the answers to three thematic units: 1) application of molecular physiological biomonitoring on aquatic organisms as a model system for monitoring of the effects of single pollutants as biomarkers of oxidative stress, 2) investigation of the effects of toxicants on antioxidant system, and the possibility of the application of antioxidants to decrease or eliminate toxic effects and 3) measurement of an adequate biochemical biomarkers of oxidative stress that may not only point to an early onset of disease, its progression and assessment of therapy effectiveness, but can also help with the clarification of the pathophysiological mechanisms of tissue damage caused by oxidative stress, as well as, prediction of disease prognosis and choice of appropiate treatment in the early stages of disease.