Molecular mechanisms of cellular responses on pathological changes in central neuronal system and peripheral organs of mammals

The proposed research project will be focused on clarifying the role of: -Purinergic signalling in the development of neurodegeneration in central nerve system (CNS) and molecular mechanisms of steroid hormones neuroprotection; -The sympathoneuronal activity and various pathways in regulation of catecholamine and antioxidative enzymes in chronic stress. -Activity, cell distribution and expression of ecto-nucleotidases, responsible for purinergic signaling termination, and particular purinoreceptor expression in animal neurodegeneration models will be examined. In pathological conditions, ecto-enzymes modulation and involvement of various signaling molecules in steroid hormone action on CNS will be explored. Research will contribute to understanding of purinergic role in neurodegeneration pathogenesis and further illumination of molecular mechanisms of steroid hormones neuroprotection in CNS. -By exploring sympathoneuronal system activity on molecular level in chronic stress conditions will illuminate the role of various pathways in the catecholamine regulation and possibilities of adequate action in pathological conditions. Tracking the gene expression changes of key enzymes involved in biosynthesis and degradation of catecholamine and adrenergic receptors, through which catecholamine achieve its effect, as well as antioxidative enzymes role can provide better insight into complex relationships of neurobiological systems and diseases caused by chronic stress.