The development of animal models of epilepsy and testing convulsive and anticonvulsive substances

Epilepsy is one of the major neurological disorders with prevalence of 1-2% and 30% of patients are refractory to available therapy. Experimental models of epilepsies are valuable for investigations of its mechanisms and therapy. NMDA receptor, key factor for induction of critical hyperexcitability, is modulated by gaseous neurotransmitters NO, H2S and CO which could modify induction and severity of seizures. Frequency and severity of epilepsy are influenced by changes in gonadal hormones during ovary cycle, menopause, puberty, as well as in polycystic ovary syndrome (form of hyperandrogenic chronic anovulation). Hepatic encephalopathy represents a complex neuropsychiatric syndrome accompanying acute or chronic liver failure. The aims of this project will be to determine the role of gaseous neurotransmitters and gonadal hormones in mechanisms of epileptogenic effects of homocysteine and lindane, by testing the effects of modulators of its levels on behavioral and EEG manifestations, as well as on oxidative status and activity of ion pumps. Contribution of various mechanisms and finasteride to the pathogenesis of hepatic encephalopathy in thioacetamide-induced acute liver failure in rats will be determined